This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pJU225 Prp8-TAP
catalog :
111305
product information
Catalog Number :
111305
Product Name :
pJU225 Prp8-TAP
article :
| doi | |
| id | 28193429 |
| pubmed_id | 8725222 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pRS424 | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | ||
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
Plasmid pJU225 (PRP8 TRPl2p) was constructed from a previously described plasmid JDYl3 (GAL::PRP8) (BROWN and BEGGS 1992). The galactose driven promoter in this plasmid was removed by cutting with NheI and XhoI. A wild-type copy of the promoter was amplified by PCR under nonmutagenic conditions and used to replace the galactose driven promoter. The entire PRP8 gene was then excised using XhoI and NotI and ligated to plasmid pRS424 (TRPl 2p) also cut with XhoI and NotI.
growth strain :
Prp8-TAP
origin :
37
pi :
|
resistance markers :
| 3357 |
tags :
Low Copy
terms :
| TRP1 |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments