pbabe-c-mycT58A+HRasG12V | Addgene 11130 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pbabe-c-mycT58A+HRasG12V

catalog :

11130

citations: 3

Reference
Chen Z, Lu T, Huang L, Wang Z, Yan Z, Guan Y, et al. Circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF. J Clin Invest. 2021;131: pubmed publisher
Toledo C, Herman J, Olsen J, Ding Y, Corrin P, Girard E, et al. BuGZ is required for Bub3 stability, Bub1 kinetochore function, and chromosome alignment. Dev Cell. 2014;28:282-94 pubmed publisher
Kendall S, Linardic C, Adam S, Counter C. A network of genetic events sufficient to convert normal human cells to a tumorigenic state. Cancer Res. 2005;65:9824-8 pubmed

product information

Catalog Number :

11130

Product Name :

pbabe-c-mycT58A+HRasG12V

article :

doi	10.1158/0008-5472.CAN-05-1543
id	415
pubmed_id	16267004

bacterial resistance :

Ampicillin

cloning :

backbone

pbabe

backbone_mutation

backbone_origin

backbone_size

4500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

Contains mouse c-myc and human Hras. This plasmid was generated by cloning c-MycT58A cDNA with flanking BamHI-EcoRI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site H-RasG12V cDNA in which HindIII/ClaI sites were again added by PCR.

origin :

pi :

alt_names

c-myc

HRas

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI(c-myc), ClaI(H-Ras)
cloning_site_5	BamHI(c-myc), HindIII(H-Ras)
promoter
sequencing_primer_3
sequencing_primer_5	pBABE 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
gene	HRAS
id	3265

aliases	Myc2, Niard, Nird, bHLHe39
gene	Myc
id	17869

genbank_ids

mutation

c-myc T58A, A156T, and G438R; and H-Ras G12V

name

c-myc, HRas

shRNA_sequence

size

2300

species

9606

Homo sapiens

10090

Mus musculus