pbabe-cyclinD1+CDK4R24C | Addgene 11129 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pbabe-cyclinD1+CDK4R24C

catalog :

11129

citations: 5

Reference
Weiand M, Ballmaier P, Niemietz C, Schmidt H, Zibert A. Combined transgene immortalized urothelial cells capable of reprogramming and hepatic differentiation. Biochem Biophys Rep. 2022;31:101308 pubmed publisher
Giordano F, Montalto F, Panno M, Ando S, De Amicis F. A Notch inhibitor plus Resveratrol induced blockade of autophagy drives glioblastoma cell death by promoting a switch to apoptosis. Am J Cancer Res. 2021;11:5933-5950 pubmed
Shin Y, Park S, Jung Y, Kim Y, Kim K, Park O, et al. Nanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteins. Sci Rep. 2015;5:14269 pubmed publisher
Toledo C, Herman J, Olsen J, Ding Y, Corrin P, Girard E, et al. BuGZ is required for Bub3 stability, Bub1 kinetochore function, and chromosome alignment. Dev Cell. 2014;28:282-94 pubmed publisher
Kendall S, Linardic C, Adam S, Counter C. A network of genetic events sufficient to convert normal human cells to a tumorigenic state. Cancer Res. 2005;65:9824-8 pubmed

product information

Catalog Number :

11129

Product Name :

pbabe-cyclinD1+CDK4R24C

article :

doi	10.1158/0008-5472.CAN-05-1543
id	415
pubmed_id	16267004

bacterial resistance :

Ampicillin

cloning :

backbone

pbabe

backbone_mutation

backbone_origin

backbone_size

4500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

This plasmid was generated by cloning cyclinD1 cDNA with flanking EcoRI-SalI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site CDK4R24C cDNA in which HindIII/ClaI sites were again added by PCR.

origin :

pi :

alt_names

cyclin D1, CDK4

cloning

clone_method	Restriction Enzyme
cloning_site_3	Sal I and Cla I
cloning_site_5	EcoRI and Hind III
promoter
sequencing_primer_3
sequencing_primer_5	pBABE 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCL1, D11S287E, PRAD1, U21B31
gene	CCND1
id	595

aliases	CMM3, PSK-J3
gene	CDK4
id	1019

genbank_ids

mutation

CDK4 R24C

name

Cyclin D1, Cyclin Dependent Kinase 4

shRNA_sequence

size

1800

species

9606

Homo sapiens