pET-30a(+)-Syk-tSH2-FX | Addgene 111272 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET-30a(+)-Syk-tSH2-FX

catalog :

111272

citations: 2

Reference
Feng C, Roy A, Post C. Entropic allostery dominates the phosphorylation-dependent regulation of Syk tyrosine kinase release from immunoreceptor tyrosine-based activation motifs. Protein Sci. 2018;27:1780-1796 pubmed publisher
Feng C, Post C. Insights into the allosteric regulation of Syk association with receptor ITAM, a multi-state equilibrium. Phys Chem Chem Phys. 2016;18:5807-18 pubmed publisher

product information

Catalog Number :

111272

Product Name :

pET-30a(+)-Syk-tSH2-FX

article :

doi	10.1039/c5cp05417f
id	28193423
pubmed_id	26468009

bacterial resistance :

Kanamycin

cloning :

backbone

pET-30a(+)

backbone_mutation

backbone_origin

Novagen

backbone_size

5422

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

MSANHLTYFFGNITREEAEDYLVQGGMTDGLYLLRQSRN
YLGGFALSVAHNRKAHHYTIERELNGTYAISGGRAHASP
ADLCHYHSQEPDGLICLLKKPFNRPPGVQPKTGPGGSGG
SGGSGSGGSGGSGGSEKMPWFHGNISRDESEQTVLIGSK
TNGKFLIRARDNSGSYALCLLHEGKVLHYRIDRDKTGKL
SIPEGKKFDTLWQLVEHYSYKPDGLLRVLTVPCQKIGAQ
encoding the following protein sequence:

growth strain :

express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3, in E.coli strain Rosetta 2 (DE3)

origin :

pi :

alt_names

Spleen tyrosine kinase

cloning

clone_method	Restriction Enzyme
cloning_site_3	Xho I
cloning_site_5	Nde I
promoter	T7 promoter
sequencing_primer_3	T7 terminator
sequencing_primer_5	T7 promoter
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Sykb
gene	Syk
id	20963

genbank_ids

NP_035648

mutation

interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3

name

murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20aa linker

shRNA_sequence

size

714

species

10090

Mus musculus