This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPICZc-ScAct1*-thymosinB-8His
catalog :
111148
citations: 1
Reference
Hatano T, Alioto S, Roscioli E, Palani S, Clarke S, Kamnev A, et al. Rapid production of pure recombinant actin isoforms in Pichia pastoris. J Cell Sci. 2018;131: pubmed publisher
product information
Catalog Number :
111148
Product Name :
pPICZc-ScAct1*-thymosinB-8His
article :
doi10.1242/jcs.213827
id28193397
pubmed_id29535210
bacterial resistance :
Bleocin (Zeocin)
cloning :
backbonepPICZc
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
Other
Pichia pastoris integration plasmid
growth strain :
Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37
growth temp :
Use LB low salt
origin :
37
pi :
alt_names
Yeast actin
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5EcoRI
promoterAOX1
sequencing_primer_3GCAAATGGCATTCTGACATCC
sequencing_primer_5GACTGGTTCCAATTGACAAGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYFL039C, ABY1, END7
geneACT1
id850504
genbank_ids
mutation
nameAct1
shRNA_sequence
size1125
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on backbone
tagThymosin beta and a His-tag
resistance markers :
2580
tags :
High Copy
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA