This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPICZc-HsACTB*-thymosinB-8His
catalog :
111146
citations: 2
Reference
Schwebach C, Kudryashova E, Agrawal R, Zheng W, Egelman E, Kudryashov D. Allosteric regulation controls actin-bundling properties of human plastins. Nat Struct Mol Biol. 2022;29:519-528 pubmed publisher
Hatano T, Alioto S, Roscioli E, Palani S, Clarke S, Kamnev A, et al. Rapid production of pure recombinant actin isoforms in Pichia pastoris. J Cell Sci. 2018;131: pubmed publisher
product information
Catalog Number :
111146
Product Name :
pPICZc-HsACTB*-thymosinB-8His
article :
doi10.1242/jcs.213827
id28193397
pubmed_id29535210
bacterial resistance :
Bleocin (Zeocin)
cloning :
backbonepPICZc
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
Other
Pichia pastoris integration
growth strain :
Expresses human beta-actin fused with thymosin beta and a His tag.
growth temp :
Use LB low salt
origin :
37
pi :
alt_names
Non-muscle beta-actin
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5EcoRI
promoterAOX1
sequencing_primer_3GCAAATGGCATTCTGACATCC
sequencing_primer_5GACTGGTTCCAATTGACAAGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
geneACTB
id60
genbank_ids
mutation
nameACTB
shRNA_sequence
size1125
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagThymosin beta and a His-tag
plasmid copy :
Prof. Robert C. Robinson
resistance markers :
2580
tags :
High Copy
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA