This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCAG-i53-bpA-EF1BFP
catalog :
111145
citations: 2
Reference
Wienert B, Nguyen D, Guenther A, Feng S, Locke M, Wyman S, et al. Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair. Nat Commun. 2020;11:2109 pubmed publisher
Yumlu S, Bashir S, Stumm J, Kühn R. Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9. Methods Mol Biol. 2019;1961:137-151 pubmed publisher
product information
Catalog Number :
111145
Product Name :
pCAG-i53-bpA-EF1BFP
article :
doi10.1007/978-1-4939-9170-9_10
id28193396
pubmed_id30912045
bacterial resistance :
Ampicillin
cloning :
backboneBluescript
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Amplified from Addgene plasmid 74939 deposited by Daniel Durocher.
growth strain :
Expression of i53 and a BFP reporter in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3MluI
cloning_site_5PacI
promoterCAG promoter
sequencing_primer_3M13 forward
sequencing_primer_5M13 reverse
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHLAC_RS03120, Hlac_0629
geneHLAC_RS03120
id7401764
genbank_ids
AAA72608.1
mutationhuman Ubiquitin, contains 8 point mutations
namei53
shRNA_sequence
size225
species
100
Synthetic
tags
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AsiSI
promoterEF1
sequencing_primer_3M13 forward
sequencing_primer_5M13 reverse
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneBFP
id449297
genbank_ids
mutation
nameBFP
shRNA_sequence
size720
species
100
Synthetic
tags
plasmid copy :
Amplified from Addgene plasmid 74939 deposited by Daniel Durocher
resistance markers :
1600
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA