This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCFD3-ovoD1
catalog :
111142
citations: 2
Reference
Götze K, Mrestani A, Beckmann P, Krohn K, Le Duc D, Velluva A, et al. Improving one-step scarless genome editing in Drosophila melanogaster by combining ovoD co-CRISPR selection with sgRNA target site masking. Biol Methods Protoc. 2022;7:bpac003 pubmed publisher
Ewen Campen B, Perrimon N. ovoD Co-selection: A Method for Enriching CRISPR/Cas9-Edited Alleles in Drosophila. G3 (Bethesda). 2018;8:2749-2756 pubmed publisher
product information
Catalog Number :
111142
Product Name :
pCFD3-ovoD1
article :
doi10.1534/g3.118.200498
id28193390
pubmed_id29934375
bacterial resistance :
Ampicillin
cloning :
backbonepCFD3
backbone_mutation
backbone_origin
backbone_size6230
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
CRISPR
growth strain :
Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoterU6:3
sequencing_primer_3
sequencing_primer_5TCGAAAATTTTTGAATAGCCCAGGT
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesDmel_CG6824, CG15467, CG6824, Dmel CG6824, Fs(1)K1103, Fs(1)K1237, Fs(1)K155, OVO, Ovo, Ovo-D, Shv, Svb, Svb/Ovo, fs(1)K1237, fs(1)M1, fs(1)M38, ovo/shavenbaby, ovo/svb, ovoD, svb, svb/ovo
geneovo
id31429
genbank_ids
mutation
namepCFD3-ovoD1
shRNA_sequenceAAAAAGAGATGCCCGCAGAG
size
species
7227
Drosophila melanogaster
tags
resistance markers :
1275
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA