This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCT310
catalog :
111140
citations: 1
Reference
Tsui C, Inouye C, Levy M, Lu A, Florens L, Washburn M, et al. dCas9-targeted locus-specific protein isolation method identifies histone gene regulators. Proc Natl Acad Sci U S A. 2018;115:E2734-E2741 pubmed publisher
product information
Catalog Number :
111140
Product Name :
pCT310
article :
doi10.1073/pnas.1718844115
id28193360
pubmed_id29507191
bacterial resistance :
Ampicillin
cloning :
backbonepET302/NT-His
backbone_mutation
backbone_originThermo Fisher (Invitrogen)
backbone_size5712
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression of 6His-dCas9-3XFlag
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3pFN Flexi REVseq / CTTTCGGGCTTTGTTAGCAG
sequencing_primer_55'_pET / CGATCCCGCGAAATTAATACGA
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationD10A - H840A
namedCas9
shRNA_sequence
size4101
species
32644
Other
Streptococcus pyogenes
tags
locationN terminal on insert
tag6X His
locationC terminal on insert
tag3X Flag
plasmid copy :
Frank Xie, Tjian Lab - Introduced D10A and H840A mutations to wild type SpCas9 (gift of the Doudna Lab)
resistance markers :
527
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA