This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSEMS-Tom20-Halo7Tag
catalog :
111135
citations: 3
Reference
Najt C, Adhikari S, Heden T, Cui W, Gansemer E, Rauckhorst A, et al. Organelle interactions compartmentalize hepatic fatty acid trafficking and metabolism. Cell Rep. 2023;42:112435 pubmed publisher
Wang B, Zhao Z, Xiong M, Yan R, Xu K. The endoplasmic reticulum adopts two distinct tubule forms. Proc Natl Acad Sci U S A. 2022;119:e2117559119 pubmed publisher
Appelhans T, Richter C, Wilkens V, Hess S, Piehler J, Busch K. Nanoscale organization of mitochondrial microcompartments revealed by combining tracking and localization microscopy. Nano Lett. 2012;12:610-6 pubmed publisher
product information
Catalog Number :
111135
Product Name :
pSEMS-Tom20-Halo7Tag
article :
doi10.1021/nl203343a
id28193387
pubmed_id22201267
bacterial resistance :
Ampicillin
cloning :
backbonepSEMS(26m)
backbone_mutationoriginal snap-Tag substituted by Halo7-Tag
backbone_originCovalys, NEB
backbone_size5325
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Tom20 with selflabeling tag, e.g. for fluorescence microscopy
origin :
37
pi :
alt_names
Tom20
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRV
promoterCMV
sequencing_primer_3TTCCACATCATCTTCAGCCAAG
sequencing_primer_5ATGGTGGGTCGGAACAGCGCCAT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMAS20, MOM19, TOM20
geneTOMM20
id9804
genbank_ids
mutation
nameReceptor of tranlocase of outer mitochondrial membrane Tom20
shRNA_sequence
size435
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHalo7 Tag
resistance markers :
3813
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA