This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CHT1-COMP-blac-flag-his
catalog :
110995
citations: 1
Reference
Kn xf6 ckel J, Dundas K, Yang A, Galaway F, Metcalf T, Gemert G, et al. Systematic Identification of Plasmodium Falciparum Sporozoite Membrane Protein Interactions Reveals an Essential Role for the p24 Complex in Host Infection. Mol Cell Proteomics. 2021;20:100038 pubmed publisher
product information
Catalog Number :
110995
Product Name :
CHT1-COMP-blac-flag-his
article :
doi10.1074/mcp.RA120.002432
id28193369
pubmed_id33515807
bacterial resistance :
Ampicillin
cloning :
backbonepTT3
backbone_mutation
backbone_originpTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
backbone_size7661
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
origin :
37
pi :
alt_names
PF3D7_1252200
PFCHT1
cloning
clone_methodRestriction Enzyme
cloning_site_3Asc1
cloning_site_5Not1
promoterCMV
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPF3D7_1252200, PFL2510w
genePF3D7_1252200
id811552
genbank_ids
mutationAll NXT/S sites mutated to NXA, where X is any amino acid except Proline
namechitinase (CHT1)
shRNA_sequence
size1134
species
tags
locationN terminal on insert
tagExogenous signal peptide of mouse origin
locationC terminal on insert
tagrat Cd4d3+4, beta-lactamase, triple FLAG tag, 6xHis tag
resistance markers :
919
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA