This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
plko-tet-on-puromycin-shTRX1-259
catalog :
110920
citations: 1
Reference
Samaranayake G, Troccoli C, Huynh M, Lyles R, Kage K, Win A, et al. Thioredoxin-1 protects against androgen receptor-induced redox vulnerability in castration-resistant prostate cancer. Nat Commun. 2017;8:1204 pubmed publisher
product information
Catalog Number :
110920
Product Name :
plko-tet-on-puromycin-shTRX1-259
article :
doi10.1038/s41467-017-01269-x [pii]
id28193358
pubmed_id29089489
bacterial resistance :
Ampicillin
cloning :
backbonepLKO-Tet-on (puromycin)
backbone_mutation
backbone_originPUBMED ID: 19177017
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
The Rai lab uses XL-10 gold ultracompetent cells as the growth strain, but DH5alpha should work fine too
growth strain :
To knockdown Thioredoxin-1 following doxycycline addition
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoterunknown
sequencing_primer_3unknown
sequencing_primer_5unknown
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesTRDX, TRX, TRX1, TXN1, Trx80
geneTXN
id7295
genbank_ids
mutation
nameTRX1
shRNA_sequenceGCTTCAGAGTGTGAAGTCAAA
size
species
9606
Homo sapiens
tags
resistance markers :
2221
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA