This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDEST-HisMBP
catalog :
11085
citations: 20
Reference
Choi S, Prabhakar P, Chowdhury R, Pendergast Iv T, Urbanowicz B, Maranas C, et al. A single amino acid change led to structural and functional differentiation of PvHd1 to control flowering in switchgrass. J Exp Bot. 2023;: pubmed publisher
Amici D, Cingoz H, Alasady M, Alhayek S, Phoumyvong C, Sahni N, et al. The HAPSTR2 retrogene buffers stress signaling and resilience in mammals. Nat Commun. 2023;14:152 pubmed publisher
Rebholz Z, Lancaster J, Larose H, Khrimian A, Luck K, Sparks M, et al. Ancient origin and conserved gene function in terpene pheromone and defense evolution of stink bugs and hemipteran insects. Insect Biochem Mol Biol. 2023;152:103879 pubmed publisher
Zhao H, Sun J, Insinna C, Lu Q, Wang Z, Nagashima K, et al. Male infertility-associated Ccdc108 regulates multiciliogenesis via the intraflagellar transport machinery. EMBO Rep. 2022;23:e52775 pubmed publisher
Cuevas Velazquez C, Vellosillo T, Guadalupe K, Schmidt H, Yu F, Moses D, et al. Intrinsically disordered protein biosensor tracks the physical-chemical effects of osmotic stress on cells. Nat Commun. 2021;12:5438 pubmed publisher
Tovey C, Tsuji C, Egerton A, Bernard F, Guichet A, de la Roche M, et al. Autoinhibition of Cnn binding to γ-TuRCs prevents ectopic microtubule nucleation and cell division defects. J Cell Biol. 2021;220: pubmed publisher
Araya Flores J, Miranda S, Covarrubias M, Stange C, Handford M. Solanum lycopersicum (tomato) possesses mitochondrial and plastidial lipoyl synthases capable of increasing lipoylation levels when expressed in bacteria. Plant Physiol Biochem. 2020;151:264-270 pubmed publisher
Eftekharzadeh B, Banduseela V, Chiesa G, Martínez Cristóbal P, Rauch J, Nath S, et al. Hsp70 and Hsp40 inhibit an inter-domain interaction necessary for transcriptional activity in the androgen receptor. Nat Commun. 2019;10:3562 pubmed publisher
Natesan M, Wu S, Chen C, Jensen S, KARLOVAC N, Dyas B, et al. A smartphone-based rapid telemonitoring system for Ebola and Marburg disease surveillance. ACS Sens. 2018;: pubmed publisher
Tovey C, Tubman C, Hamrud E, Zhu Z, Dyas A, Butterfield A, et al. ?-TuRC Heterogeneity Revealed by Analysis of Mozart1. Curr Biol. 2018;28:2314-2323.e6 pubmed publisher
De Mol E, Szulc E, Di Sanza C, Martínez Cristóbal P, Bertoncini C, Fenwick R, et al. Regulation of Androgen Receptor Activity by Transient Interactions of Its Transactivation Domain with General Transcription Regulators. Structure. 2018;26:145-152.e3 pubmed publisher
Rajan A, Housden B, Wirtz Peitz F, Holderbaum L, Perrimon N. A Mechanism Coupling Systemic Energy Sensing to Adipokine Secretion. Dev Cell. 2017;43:83-98.e6 pubmed publisher
Raran Kurussi S, Waugh D. Expression and Purification of Recombinant Proteins in Escherichia coli with a His6 or Dual His6-MBP Tag. Methods Mol Biol. 2017;1607:1-15 pubmed publisher
Chattopadhyay D, Swingle M, Salter E, Wood E, D Arcy B, Zivanov C, et al. Crystal structures and mutagenesis of PPP-family ser/thr protein phosphatases elucidate the selectivity of cantharidin and novel norcantharidin-based inhibitors of PP5C. Biochem Pharmacol. 2016;109:14-26 pubmed publisher
Raines T, Shanks C, Cheng C, McPherson D, Argueso C, Kim H, et al. The cytokinin response factors modulate root and shoot growth and promote leaf senescence in Arabidopsis. Plant J. 2016;85:134-47 pubmed publisher
Kamata T, Natesan M, Warfield K, Aman M, Ulrich R. Determination of specific antibody responses to the six species of ebola and Marburg viruses by multiplexed protein microarrays. Clin Vaccine Immunol. 2014;21:1605-12 pubmed publisher
Im Y, Brglez I, Dieck C, Perera I, Boss W. Phosphatidylinositol 4-kinase and phosphatidylinositol 4-phosphate 5-kinase assays. Methods Mol Biol. 2013;1009:163-74 pubmed publisher
Wong M, Jiang S, Palasingam P, Kolatkar P. A divalent ion is crucial in the structure and dominant-negative function of ID proteins, a class of helix-loop-helix transcription regulators. PLoS ONE. 2012;7:e48591 pubmed publisher
Fu Z, Yan S, Saleh A, Wang W, Ruble J, Oka N, et al. NPR3 and NPR4 are receptors for the immune signal salicylic acid in plants. Nature. 2012;486:228-32 pubmed publisher
Nallamsetty S, Austin B, Penrose K, Waugh D. Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli. Protein Sci. 2005;14:2964-71 pubmed
product information
Catalog Number :
11085
Product Name :
pDEST-HisMBP
article :
doi10.1110/ps.051718605
id464
pubmed_id16322578
bacterial resistance :
Chloramphenicol and Ampicillin
cloning :
backbonepMal-C2
backbone_mutation
backbone_originNew England Biolabs
backbone_size6000
promotertac promoter
sequencing_primer_3M13_pUC_fwd; M13-F20
sequencing_primer_5MBP-F
vector_types
Bacterial Expression
growth notes :
The MBP ORF can be sequenced using 5'-GAG CGG ATA ACA ATT TCA CAC AGG-3' as a tac promoter sequencing primer (in the forward direction). To sequence inserts fused to the C-terminus of MBP, the primers are 5'-GAT GAA GCC CTG AAA GAC GCG CAG-3' (forward) and 5'-GCA AGG CGA TTA AGT TGG GTA ACG C-3' (reverse).
growth temp :
plasmid must be grown in gyrA mutant resistant to the toxin CcdB
origin :
37
resistance markers :
565
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA