This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLVX-SBP-mGFP-COL1A1
catalog :
110726
citations: 5
Reference
Mansur A, Joseph R, Kim E, Jean Beltran P, Udeshi N, Pearce C, et al. Dynamic regulation of inter-organelle communication by ubiquitylation controls skeletal muscle development and disease onset. elife. 2023;12: pubmed publisher
Hazari Y, Urra H, Garcia Lopez V, Diaz J, Tamburini G, Milani M, et al. The endoplasmic reticulum stress sensor IRE1 regulates collagen secretion through the enforcement of the proteostasis factor P4HB/PDIA1 contributing to liver damage and fibrosis. bioRxiv. 2023;: pubmed publisher
Buchl S, Hanquier Z, Haak A, Thomason Y, Huebert R, Shah V, et al. Traf2 and NCK Interacting Kinase Is a Critical Regulator of Procollagen I Trafficking and Hepatic Fibrogenesis in Mice. Hepatol Commun. 2021;: pubmed publisher
Stevenson N, Bergen D, Lu Y, Prada Sanchez M, Kadler K, Hammond C, et al. Giantin is required for intracellular N-terminal processing of type I procollagen. J Cell Biol. 2021;220: pubmed publisher
McCaughey J, Stevenson N, Cross S, Stephens D. ER-to-Golgi trafficking of procollagen in the absence of large carriers. J Cell Biol. 2019;218:929-948 pubmed publisher
product information
Catalog Number :
110726
Product Name :
pLVX-SBP-mGFP-COL1A1
article :
doi10.1083/jcb.201806035
id28193306
pubmed_id30587510
bacterial resistance :
Ampicillin
cloning :
backbonepLVX-Puro
backbone_mutationNone other than cloning of insert
backbone_originClontech
backbone_size8102
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
See attached PDF for full descritions
growth strain :
2nd generation lentiviral transfer plasmid. Expresses an engineered human procollagen type I alpha 1 with SBP and mGFP between the pro sequence and triple helical region
origin :
37
pi :
alt_names
mGFP-procollagen
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3GATGGCCACATCGGCAG
sequencing_primer_5CGC AAA TGG GCG GTA GGC GTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4
geneCOL1A1
id1277
genbank_ids
AAB94054.3
P02452.5
mutationSee attached PDF
namePro-SBP-mGFP-COL1A1
shRNA_sequence
size
species
9606
Homo sapiens
100
Synthetic
32644
Other
tags
location
tagmGFP
location
tagSBP: streptavidin binding peptide
resistance markers :
2402
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA