This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSKC12 - DelQ
catalog :
110493
citations: 1
Reference
Kumari S, Bugaut A, Huppert J, Balasubramanian S. An RNA G-quadruplex in the 5' UTR of the NRAS proto-oncogene modulates translation. Nat Chem Biol. 2007;3:218-21 pubmed
product information
Catalog Number :
110493
Product Name :
pSKC12 - DelQ
article :
doi10.1038/nchembio864
id28193258
pubmed_id17322877
bacterial resistance :
Ampicillin
cloning :
backbonepUC18
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Luciferase
growth strain :
Monitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoterT7
sequencing_primer_3M13-rev
sequencing_primer_5M13-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6
geneNRAS
id4893
genbank_ids
AM489487
mutationDeletion of the first 29 base pairs of the NRAS 5 UTR (sequence comprising the RNA G-quadruplex-forming sequence)
nameNRAS 5'- DelQ (bp 30-254)
shRNA_sequence
size224
species
9606
Homo sapiens
tags
locationC terminal on insert
tagFirefly luciferase
resistance markers :
1998
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA