pcDNA3 HRPT2 136X | Addgene 11049 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3 HRPT2 136X

catalog :

11049

citations: 1

Reference
Rozenblatt Rosen O, Hughes C, Nannepaga S, Shanmugam K, Copeland T, Guszczynski T, et al. The parafibromin tumor suppressor protein is part of a human Paf1 complex. Mol Cell Biol. 2005;25:612-20 pubmed

product information

Catalog Number :

11049

Product Name :

pcDNA3 HRPT2 136X

article :

doi	10.1128/MCB.25.2.612-620.2005
id	445
pubmed_id	15632063

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Author has deposited insert sequence. Click on sequence to view. Cloned using the following primers 5' CGGGATCCATGGCGGACGTGCTTAGCGT 3' and 5' CCGCTCGAGCTATGCTTCTGCTAAAACTTC 3' and ligated into pcDNA3 HRPT2 that had been digested with BamHI & XhoI (which removes full-length HRPT2, but leaves the flag tag at the N-terminus. See plasmid 11048 to learn how pcDNA3 HRPT2 was constructed).

origin :

pi :

alt_names

HRPT2

parafibromin

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	BamHI
promoter
sequencing_primer_3	SP6
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C1orf28, FIHP, HPTJT, HRPT1, HRPT2, HYX
gene	CDC73
id	79577

genbank_ids

mutation

truncation mutant with a stop codon introduced at aa136

name

HRPT2 136X

shRNA_sequence

size

136

species

9606

Homo sapiens