This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLKO.1-TRC.mKO2_shHRASLS.1
catalog :
110323
citations: 1
Reference
Quintero M, Adamoski D, Reis L, Ascenção C, Oliveira K, Gonçalves K, et al. Guanylate-binding protein-1 is a potential new therapeutic target for triple-negative breast cancer. BMC Cancer. 2017;17:727 pubmed publisher
product information
Catalog Number :
110323
Product Name :
pLKO.1-TRC.mKO2_shHRASLS.1
article :
doi10.1186/s12885-017-3726-2 [pii]
id28193230
pubmed_id29115931
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1-TRC.mKO2
backbone_mutation
backbone_origin#85208
backbone_size7032
promoterRNA polymerase III promoter for human U6 snRNA for shRNA and hPGK promoter for mKO2
sequencing_primer_3
sequencing_primer_5GACTATCATATGCTTACCGT
vector_types
Mammalian Expression
Lentiviral
RNAi
growth notes :
See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors. Plasmid grows more slowly than standard plasmids.
growth strain :
TRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2
origin :
37
pi :
alt_names
HRAS like suppressor
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoterU6 (RNA Pol III)
sequencing_primer_3
sequencing_primer_5GACTATCATATGCTTACCGT
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesA-C1, H-REV107, HRASLS, HRASLS1, HRSL1, HSD28, PLA/AT1, PLAAT-1
genePLAAT1
id57110
genbank_ids
57110
mutation
nameHRASLS
shRNA_sequenceGATAAGTACCGTTGAGTTTG
size
species
9606
Homo sapiens
32644
Other
Homo sapiens
tags
resistance markers :
3140
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA