This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
EGFR D770-N771 insNPG
catalog :
11016
citations: 5
Reference
Deygas M, Gadet R, Gillet G, Rimokh R, Gonzalo P, Mikaelian I. Redox regulation of EGFR steers migration of hypoxic mammary cells towards oxygen. Nat Commun. 2018;9:4545 pubmed publisher
Sancho Martinez I, Nivet E, Xia Y, Hishida T, Aguirre A, Ocampo A, et al. Establishment of human iPSC-based models for the study and targeting of glioma initiating cells. Nat Commun. 2016;7:10743 pubmed publisher
Whitsett T, CHENG E, Inge L, Asrani K, Jameson N, Hostetter G, et al. Elevated expression of Fn14 in non-small cell lung cancer correlates with activated EGFR and promotes tumor cell migration and invasion. Am J Pathol. 2012;181:111-20 pubmed publisher
Rossi M, Colecchia D, Iavarone C, Strambi A, Piccioni F, Verrotti di Pianella A, et al. Extracellular signal-regulated kinase 8 (ERK8) controls estrogen-related receptor ? (ERR?) cellular localization and inhibits its transcriptional activity. J Biol Chem. 2011;286:8507-22 pubmed publisher
Greulich H, Chen T, Feng W, Janne P, Alvarez J, Zappaterra M, et al. Oncogenic transformation by inhibitor-sensitive and -resistant EGFR mutants. PLoS Med. 2005;2:e313 pubmed
product information
Catalog Number :
11016
Product Name :
EGFR D770-N771 insNPG
article :
doi10.1371/journal.pmed.0020313
id455
pubmed_id16187797
bacterial resistance :
Ampicillin
cloning :
backbonepBABE puro
backbone_mutation
backbone_originavailable at Addgene
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Author has deposited the sequence of the insert to Addgene. Click on sequence to view. Mutation made by using the following primers to PCR from plasmid 11011: 5'-GGCCAGCGTGGACAACCCCGGCAACCCCCACGT-3' and 5'-ACGTGGGGGTTGCCGGGGTTGTCCACGCTGGCC-3'
growth strain :
Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells
origin :
37
pi :
alt_names
epidermal growth factor receptor
ERBB
cloning
clone_methodRestriction Enzyme
cloning_site_3SnaBI
cloning_site_5SnaBI
promoter
sequencing_primer_3
sequencing_primer_5pBABE5'
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA
geneEGFR
id1956
genbank_ids
mutationD770-N771 insNPG
nameEGFR D770-N771 insNPG
shRNA_sequence
size3648
species
9606
Homo sapiens
tags
resistance markers :
338
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA