This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
EGFR G719S
catalog :
11013
citations: 1
Reference
Greulich H, Chen T, Feng W, Janne P, Alvarez J, Zappaterra M, et al. Oncogenic transformation by inhibitor-sensitive and -resistant EGFR mutants. PLoS Med. 2005;2:e313 pubmed
product information
Catalog Number :
11013
Product Name :
EGFR G719S
article :
doi10.1371/journal.pmed.0020313
id455
pubmed_id16187797
bacterial resistance :
Ampicillin
cloning :
backbonepBABE puro
backbone_mutation
backbone_originavailable at Addgene
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Author has deposited the sequence of the insert to Addgene. Click on sequence to view. Point mutation made by using the following primers to PCR from plasmid 11011: 5'-AAGATCAAAGTGCTGAGCTCCGGTGCGTTCG-3' and 5'-CGAACGCACCGGAGCTCAGCACTTTGATCTT-3'
growth strain :
Retroviral construct for expressing human EGFR with G719S mutation in human cells
origin :
37
pi :
alt_names
epidermal growth factor receptor
ERBB
cloning
clone_methodRestriction Enzyme
cloning_site_3SnaBI
cloning_site_5SnaBI
promoter
sequencing_primer_3
sequencing_primer_5pBABE5'
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA
geneEGFR
id1956
genbank_ids
mutationG719S
nameEGFR G719S
shRNA_sequence
size3639
species
9606
Homo sapiens
tags
resistance markers :
338
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA