This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Hy_pMT Flag-Hel25E D193E
catalog :
110120
citations: 1
Reference
Huang C, Liang D, Tatomer D, Wilusz J. A length-dependent evolutionarily conserved pathway controls nuclear export of circular RNAs. Genes Dev. 2018;32:639-644 pubmed publisher
product information
Catalog Number :
110120
Product Name :
Hy_pMT Flag-Hel25E D193E
article :
doi10.1101/gad.314856.118
id28193182
pubmed_id29773557
bacterial resistance :
Ampicillin
cloning :
backboneHy_pMT
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth strain :
Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5AgeI
promoterMetallothionein Promoter (pMT)
sequencing_primer_3
sequencing_primer_5CACTCGAATTTGGAGCCGGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG7269, CG7269, Dbp25F, DmRH4, Dmel CG7269, HEL, HEL25E, Hel, UAP56, Uap56, WM6, Wm6, dHel25E, hel, jf26, l(2)25Eb, l(2)gdh-12, l(2)gdh12, l(2)jf26, l(2)k11511, sz, uap56
geneHel25E
id33781
genbank_ids
mutationD193E mutation
nameHel25E
shRNA_sequence
size
species
7227
Drosophila melanogaster
tags
locationN terminal on backbone
tagFlag
resistance markers :
2108
tags :
Unknown
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA