This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
EGFR L858R
catalog :
11012
citations: 17
Reference
Tsutsumi H, Iwama E, Ibusuki R, Shimauchi A, Ota K, Yoneshima Y, et al. Mutant forms of EGFR promote HER2 trafficking through efficient formation of HER2-EGFR heterodimers. Lung Cancer. 2023;175:101-111 pubmed publisher
Prieto Garcia C, Hartmann O, Reissland M, Braun F, Bozkurt S, Pahor N, et al. USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K. Mol Oncol. 2022;: pubmed publisher
Song X, Tang W, Peng H, Qi X, Li J. FGFR leads to sustained activation of STAT3 to mediate resistance to EGFR-TKIs treatment. Invest New Drugs. 2021;: pubmed publisher
Nishiya N, Oku Y, Ishikawa C, Fukuda T, Dan S, Mashima T, et al. Lamellarin 14, a derivative of marine alkaloids, inhibits the T790M/C797S mutant epidermal growth factor receptor. Cancer Sci. 2021;112:1963-1974 pubmed publisher
Vaishnavi A, Scherzer M, Kinsey C, Parkman G, Truong A, Ghazi P, et al. Inhibition of MEK1/2 Forestalls the Onset of Acquired Resistance to Entrectinib in Multiple Models of NTRK1-Driven Cancer. Cell Rep. 2020;32:107994 pubmed publisher
Kim D, Park S, Kim D, Jeong M, Noh J, Kwon Y, et al. Direct visualization of single-molecule membrane protein interactions in living cells. PLoS Biol. 2018;16:e2006660 pubmed publisher
Du W, Ni L, Liu B, Wei Y, Lv Y, Qiang S, et al. Upregulation of SALL4 by EGFR activation regulates the stemness of CD44-positive lung cancer. Oncogenesis. 2018;7:36 pubmed publisher
Weng Y, Fan X, Bai Y, Wang S, Huang H, Yang H, et al. SLC2A5 promotes lung adenocarcinoma cell growth and metastasis by enhancing fructose utilization. Cell Death Discov. 2018;4:38 pubmed publisher
Giuliano C, Lin A, Smith J, Palladino A, Sheltzer J. MELK expression correlates with tumor mitotic activity but is not required for cancer growth. elife. 2018;7: pubmed publisher
Song X, Qi X, Wang Q, Zhu W, Li J. A novel multi-target inhibitor harboring selectivity of inhibiting EGFR T790M sparing wild-type EGFR. Am J Cancer Res. 2017;7:1884-1898 pubmed
Xu Y, Wang X, Chen Y, Chen S, Yang X, Sun Y, et al. Integration of Receptor Tyrosine Kinases Determines Sensitivity to PI3K?-selective Inhibitors in Breast Cancer. Theranostics. 2017;7:974-986 pubmed publisher
Nakamura Y, Ohler Z, Householder D, Nagaya T, Sato K, Okuyama S, et al. Near Infrared Photoimmunotherapy in a Transgenic Mouse Model of Spontaneous Epidermal Growth Factor Receptor (EGFR)-expressing Lung Cancer. Mol Cancer Ther. 2017;16:408-414 pubmed publisher
Ku B, Bae Y, Koh J, Sun J, Lee S, Ahn J, et al. AZD9291 overcomes T790 M-mediated resistance through degradation of EGFR(L858R/T790M) in non-small cell lung cancer cells. Invest New Drugs. 2016;34:407-15 pubmed publisher
Unni A, Lockwood W, Zejnullahu K, Lee Lin S, Varmus H. Evidence that synthetic lethality underlies the mutual exclusivity of oncogenic KRAS and EGFR mutations in lung adenocarcinoma. elife. 2015;4:e06907 pubmed publisher
Whitsett T, CHENG E, Inge L, Asrani K, Jameson N, Hostetter G, et al. Elevated expression of Fn14 in non-small cell lung cancer correlates with activated EGFR and promotes tumor cell migration and invasion. Am J Pathol. 2012;181:111-20 pubmed publisher
Rossi M, Colecchia D, Iavarone C, Strambi A, Piccioni F, Verrotti di Pianella A, et al. Extracellular signal-regulated kinase 8 (ERK8) controls estrogen-related receptor ? (ERR?) cellular localization and inhibits its transcriptional activity. J Biol Chem. 2011;286:8507-22 pubmed publisher
Greulich H, Chen T, Feng W, Janne P, Alvarez J, Zappaterra M, et al. Oncogenic transformation by inhibitor-sensitive and -resistant EGFR mutants. PLoS Med. 2005;2:e313 pubmed
product information
Catalog Number :
11012
Product Name :
EGFR L858R
article :
doi10.1371/journal.pmed.0020313
id455
pubmed_id16187797
bacterial resistance :
Ampicillin
cloning :
backbonepBABE puro
backbone_mutation
backbone_originavailable at Addgene
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Author has deposited the sequence of the insert to Addgene. Click on sequence to view. Point mutation made by using the following primers to PCR from plasmid 11011: 5'-AAGATCACAGATTTTGGGAGGGCCAAACTGCTGGGTG-3' and 5'-CACCCAGCAGTTTGGCCCTCCCAAAATCTGTGATCTT-3'.
growth strain :
Retroviral construct for expressing human EGFR with L858R mutation in human cells
origin :
37
pi :
alt_names
epidermal growth factor receptor
ERBB
cloning
clone_methodRestriction Enzyme
cloning_site_3SnaBI
cloning_site_5SnaBI
promoter
sequencing_primer_3
sequencing_primer_5pBABE5'
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA
geneEGFR
id1956
genbank_ids
mutationL858R (tumor-derived)
nameEGFR L858R
shRNA_sequence
size3639
species
9606
Homo sapiens
tags
resistance markers :
338
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA