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product summary
company name :
Addgene
product type :
cDNA
product name :
pHaMDRwt
catalog :
10957
citations: 27
Reference
Lin Y, Lin J, Liu Y, Chen Y, Liu S, Hsu K, et al. USP7 Induces Chemoresistance in Triple-Negative Breast Cancer via Deubiquitination and Stabilization of ABCB1. Cells. 2022;11: pubmed publisher
Bertova A, Kontar S, Polozsanyi Z, Simkovic M, Rosenbergova Z, Rebros M, et al. Effects of Sulforaphane-Induced Cell Death upon Repeated Passage of Either P-Glycoprotein-Negative or P-Glycoprotein-Positive L1210 Cell Variants. Int J Mol Sci. 2022;23: pubmed publisher
Kyca T, Pavlíková L, Boháčová V, Misak A, Poturnayová A, Breier A, et al. Insight into Bortezomib Focusing on Its Efficacy against P-gp-Positive MDR Leukemia Cells. Int J Mol Sci. 2021;22: pubmed publisher
Waghela B, Vaidya F, Pathak C. Upregulation of NOX-2 and Nrf-2 Promotes 5-Fluorouracil Resistance of Human Colon Carcinoma (HCT-116) Cells. Biochemistry (Mosc). 2021;86:262-274 pubmed publisher
Cagala M, Pavlíková L, Sereš M, Kadlecikova K, Breier A, Sulova Z. Development of Resistance to Endoplasmic Reticulum Stress-Inducing Agents in Mouse Leukemic L1210 Cells. Molecules. 2020;25: pubmed publisher
Kontar S, Imrichova D, Bertova A, Macková K, Poturnayová A, Sulova Z, et al. Cell Death Effects Induced by Sulforaphane and Allyl Isothiocyanate on P-Glycoprotein Positive and Negative Variants in L1210 Cells. Molecules. 2020;25: pubmed publisher
Sereš M, Pavlíková L, Boháčová V, Kyca T, Borovska I, Lakatos B, et al. Overexpression of GRP78/BiP in P-Glycoprotein-Positive L1210 Cells is Responsible for Altered Response of Cells to Tunicamycin as a Stressor of the Endoplasmic Reticulum. Cells. 2020;9: pubmed publisher
Durrant D, Das A, Dyer S, Kukreja R. A dual PI3 kinase/mTOR inhibitor BEZ235 reverses doxorubicin resistance in ABCB1 overexpressing ovarian and pancreatic cancer cell lines. Biochim Biophys Acta Gen Subj. 2020;1864:129556 pubmed publisher
Hemming M, Lawlor M, Andersen J, Hagan T, Chipashvili O, Scott T, et al. Enhancer Domains in Gastrointestinal Stromal Tumor Regulate KIT Expression and Are Targetable by BET Bromodomain Inhibition. Cancer Res. 2019;79:994-1009 pubmed publisher
Einarsdottir B, Karlsson J, Söderberg E, Lindberg M, Funck Brentano E, Jespersen H, et al. A patient-derived xenograft pre-clinical trial reveals treatment responses and a resistance mechanism to karonudib in metastatic melanoma. Cell Death Dis. 2018;9:810 pubmed publisher
Elefantová K, Lakatos B, Kubickova J, Sulova Z, Breier A. Detection of the Mitochondrial Membrane Potential by the Cationic Dye JC-1 in L1210 Cells with Massive Overexpression of the Plasma Membrane ABCB1 Drug Transporter. Int J Mol Sci. 2018;19: pubmed publisher
Boháčová V, Sereš M, Pavlíková L, Kontar S, Cagala M, Bobal P, et al. Triorganotin Derivatives Induce Cell Death Effects on L1210 Leukemia Cells at Submicromolar Concentrations Independently of P-glycoprotein Expression. Molecules. 2018;23: pubmed publisher
Xia Y, Hua R, Juan C, Zhong Z, Tao C, Fang R, et al. SIRT6 Depletion Sensitizes Human Hepatoma Cells to Chemotherapeutics by Downregulating MDR1 Expression. Front Pharmacol. 2018;9:194 pubmed publisher
Cao W, Kayama H, Chen M, Delmas A, Sun A, Kim S, et al. The Xenobiotic Transporter Mdr1 Enforces T Cell Homeostasis in the Presence of Intestinal Bile Acids. Immunity. 2017;47:1182-1196.e10 pubmed publisher
Pavlíková L, Sereš M, Hano M, Boháčová V, Sevcikova I, Kyca T, et al. L1210 Cells Overexpressing ABCB1 Drug Transporters Are Resistant to Inhibitors of the N- and O-glycosylation of Proteins. Molecules. 2017;22: pubmed publisher
Nyquist M, Corella A, Burns J, Coleman I, Gao S, Tharakan R, et al. Exploiting AR-Regulated Drug Transport to Induce Sensitivity to the Survivin Inhibitor YM155. Mol Cancer Res. 2017;15:521-531 pubmed publisher
Dubach J, Kim E, Yang K, Cuccarese M, Giedt R, Meimetis L, et al. Quantitating drug-target engagement in single cells in vitro and in vivo. Nat Chem Biol. 2017;13:168-173 pubmed publisher
Pavlíková L, Sereš M, Imrichova D, Hano M, Rusnák A, Zamorova M, et al. The expression of P-gp in leukemia cells is associated with cross-resistance to protein N-glycosylation inhibitor tunicamycin. Gen Physiol Biophys. 2016;35:497-510 pubmed
Yang Y, Wu N, Wang Z, Zhang F, Tian R, Ji W, et al. Rack1 Mediates the Interaction of P-Glycoprotein with Anxa2 and Regulates Migration and Invasion of Multidrug-Resistant Breast Cancer Cells. Int J Mol Sci. 2016;17: pubmed
Coculova M, Imrichova D, Seres M, Messingerová L, Boháčová V, Sulova Z, et al. The expression of P-glycoprotein in leukemia cells is associated with the upregulated expression of nestin, a class 6 filament protein. Leuk Res. 2016;48:32-9 pubmed publisher
Sun Y, Guan Z, Liang L, Cheng Y, Zhou J, Li J, et al. HIF-1α/MDR1 pathway confers chemoresistance to cisplatin in bladder cancer. Oncol Rep. 2016;35:1549-56 pubmed publisher
Wolfram J, Scott B, Boom K, Shen J, Borsoi C, Suri K, et al. Hesperetin Liposomes for Cancer Therapy. Curr Drug Deliv. 2016;13:711-9 pubmed
Breier A, Stetka J, Boháčová V, Macejova D, Brtko J, Sulova Z. Effect of 9-cis retinoic acid and all-trans retinoic acid in combination with verapamil on P-glycoprotein expression in L1210 cells. Neoplasma. 2014;61:553-65 pubmed publisher
Li X, Hu J, Wang B, Sheng L, Liu Z, Yang S, et al. Inhibitory effects of herbal constituents on P-glycoprotein in vitro and in vivo: herb-drug interactions mediated via P-gp. Toxicol Appl Pharmacol. 2014;275:163-75 pubmed publisher
Bubenčíkova T, Cholujova D, Messingerová L, Mislovicová D, Sereš M, Breier A, et al. Detection of glycomic alterations induced by overexpression of p-glycoprotein on the surfaces of L1210 cells using sialic acid binding lectins. Int J Mol Sci. 2012;13:15177-92 pubmed publisher
Sereš M, Cholujova D, Bubenčíkova T, Breier A, Sulová Z. Tunicamycin depresses P-glycoprotein glycosylation without an effect on its membrane localization and drug efflux activity in L1210 cells. Int J Mol Sci. 2011;12:7772-84 pubmed publisher
Pastan I, Gottesman M, Ueda K, Lovelace E, Rutherford A, Willingham M. A retrovirus carrying an MDR1 cDNA confers multidrug resistance and polarized expression of P-glycoprotein in MDCK cells. Proc Natl Acad Sci U S A. 1988;85:4486-90 pubmed
product information
Catalog Number :
10957
Product Name :
pHaMDRwt
article :
doi10.1073/pnas.85.12.4486
id797
pubmed_id2898143
bacterial resistance :
Ampicillin
cloning :
backbonepHa
backbone_mutation
backbone_origin
backbone_size11000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Full-length cDNA, has the wild-type glycine at position 185 on P-glycoprotein (see GenBank ID M14758.1 for the sequence variations at nucleotides 382, 964, 978, 979, 1660 and 2065 that are present in this plasmid) The Ha in pHaMDRwt stand for the Harvey sarcoma virus. Vector can confer the complete multidrug-resistance phenotype to various drug sensitive cell lines. Figure legend for author's map: Structure of retroviral vector carrying MDR] cDNA. sites: B, BamHI; E, EcoRI; H, HindIII; S, Stu I; X, Xho I. bp, Base pairs; amp, ampicillin resistance gene.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5SacII
promoter
sequencing_primer_3
sequencing_primer_5na
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesABC20, CD243, CLCS, GP170, MDR1, P-GP, PGY1, p-170
geneABCB1
id5243
genbank_ids
M14758
mutation
nameMDR1
shRNA_sequence
size4000
species
9606
Homo sapiens
tags
resistance markers :
171
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA