ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP) | Addgene 109428 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

catalog :

109428

citations: 2

Reference
Banerjee D, Langberg K, Abbas S, Odermatt E, Yerramothu P, Volaric M, et al. A non-canonical, interferon-independent signaling activity of cGAMP triggers DNA damage response signaling. Nat Commun. 2021;12:6207 pubmed publisher
St Martin A, Salamango D, Serebrenik A, Shaban N, Brown W, Donati F, et al. A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 2018;46:e84 pubmed publisher

product information

Catalog Number :

109428

Product Name :

ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

article :

doi	10.1093/nar/gky332
id	28193157
pubmed_id	29746667

bacterial resistance :

Ampicillin

cloning :

backbone

HIV-1 NL4-3

backbone_mutation

Removed HIV-1 nef, vif, vpr, and part of env. Inserted CMV mCherry T2A GFP with a 43 base pair insertion in mCherry.

backbone_origin

backbone_size

9269

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth strain :

A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.

origin :

pi :

43 base pairs into mCherry to create reporternamemCherry T2A GFPshRNA_sequencesize1545species

100

Synthetic

tags
Enzymecloning_site_3<
/td>KpnIcloning_s
ite_5BamHIpr
omoterCMVseq
uencing_primer_3CTTGTACTCGAGAT
CTGCACCGGGCTTGTACAGCTCGTCCATGCCr>sequencing_primer_5c
tggctaccggtatggtgagcaagggcgagg>site_3_destroyed
site_5_destroyed<
/td>entr
ez_genegenba
nk_idsmutati
onInserted

alt_names

cloning

clone_method

Restriction

resistance markers :

3940

tags :

Unknown