This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEXL flag Smad3
catalog :
10920
citations: 4
Reference
Baumann B, Hayashida T, Liang X, Schnaper H. Hypoxia-inducible factor-1α promotes glomerulosclerosis and regulates COL1A2 expression through interactions with Smad3. Kidney Int. 2016;90:797-808 pubmed publisher
Bultmann I, Conradi A, Kretschmer C, Sterner Kock A. Latent transforming growth factor ?-binding protein 4 is downregulated in esophageal cancer via promoter methylation. PLoS ONE. 2013;8:e65614 pubmed publisher
Marchildon F, St Louis C, Akter R, Roodman V, Wiper Bergeron N. Transcription factor Smad3 is required for the inhibition of adipogenesis by retinoic acid. J Biol Chem. 2010;285:13274-84 pubmed publisher
Liu X, Sun Y, Constantinescu S, Karam E, Weinberg R, Lodish H. Transforming growth factor beta-induced phosphorylation of Smad3 is required for growth inhibition and transcriptional induction in epithelial cells. Proc Natl Acad Sci U S A. 1997;94:10669-74 pubmed
product information
Catalog Number :
10920
Product Name :
pEXL flag Smad3
article :
doi10.1073/pnas.94.20.10669
id425
pubmed_id9380693
bacterial resistance :
Kanamycin
cloning :
backbonepEXL (derived from pEGFP-N1)
backbone_mutation
backbone_originEGFP-N1 is from Clontech
backbone_size4750
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This is a modified version of pEGFP-N1 where the GFP is replaced with flag-Smad3.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5CMV-Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3
geneSMAD3
id4088
genbank_ids
mutation
nameSmad3
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagflag
resistance markers :
568
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA