This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
B9L-BirA*
catalog :
109146
citations: 1
Reference
van Tienen L, Mieszczanek J, Fiedler M, Rutherford T, Bienz M. Constitutive scaffolding of multiple Wnt enhanceosome components by Legless/BCL9. elife. 2017;6: pubmed publisher
product information
Catalog Number :
109146
Product Name :
B9L-BirA*
article :
doi10.7554/eLife.20882
id28192928
pubmed_id28296634
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA5/FRT/TO
backbone_mutation
backbone_originThermofisher
backbone_size5100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Gene Origin - PMID: 15574752 Note: The Bcl9I insert contains a V229A mutation compared to the closest reference sequence, NP_084532.2. The depositor has confirmed that this does not impact the function of the plasmid.
growth strain :
To generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV/TetO2
sequencing_primer_3BGH reverse
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesB9L, BC003321, BCL9-2, DLNB, DLNB11
geneBcl9l
id80288
genbank_ids
mutation
nameB9L
shRNA_sequence
size
species
10090
Mus musculus
tags
locationN terminal on insert
tagFlag
locationC terminal on insert
tagBirA(R118G)
plasmid copy :
PMID: 15574752
resistance markers :
3863
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA