AICSDP-52: HIST1H2BJ-mEGFP | Addgene 109121 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

AICSDP-52: HIST1H2BJ-mEGFP

catalog :

109121

citations: 2

Reference
Hayes B, Zhu P, Wang M, Pfeifer C, Xia Y, Phan S, et al. Confinement plus myosin-II suppression maximizes heritable loss of chromosomes, as revealed by live-cell ChReporters. J Cell Sci. 2023;136: pubmed publisher
M xf6 ller L, Aird E, Schr xf6 der M, Kobel L, Kissling L, van de Venn L, et al. Recursive Editing improves homology-directed repair through retargeting of undesired outcomes. Nat Commun. 2022;13:4550 pubmed publisher

product information

Catalog Number :

109121

Product Name :

AICSDP-52: HIST1H2BJ-mEGFP

article :

doi
id	28192802
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pUC57

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

CRISPR

Other

Donor Template

growth notes :

This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human HIST1H2BJ in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: DPPVAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .

growth strain :

Homology arms and linker-mEGFP sequence for C-terminus tagging of human HIST1H2BJ

origin :

pi :

alt_names

H2BJ

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	H2B/r, H2BFR, H2BJ, HIST1H2BJ
gene	H2BC11
id	8970

genbank_ids

mutation

homology arms contain point mutations to disrupt crRNA binding sites used

name

HIST1H2BJ Homology Arms with linker-mEGFP

shRNA_sequence

size

2732

species

9606

Homo sapiens