This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pScDD2_ERdd
catalog :
109047
product information
Catalog Number :
109047
Product Name :
pScDD2_ERdd
article :
doi
id28193068
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepSc with SOD1 promoter
backbone_mutation
backbone_origin
backbone_size6292
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).
origin :
37
pi :
alt_names
ERLBD
ERS1 ligand binding domain
cloning
clone_methodRestriction Enzyme
cloning_site_3SacII
cloning_site_5SmaI
promoterSOD1
sequencing_primer_3TAGACAAGCCGACAACCTTG ( in ADH1 terminator)
sequencing_primer_5GCTGTAACTATGTTGCGGAA ( in SOD promoter) or CCTTATCCAAAGATCCAAACG ( in yeGFP)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesER, ESR, ESRA, ESTRR, Era, NR3A1
geneESR1
id2099
genbank_ids
BC128573.1
mutation3 mutations : E380G, R434G, N532S
nameER50
shRNA_sequence
size1473
species
32644
Other
H. sapiens gene with yeast enhanced codon
tags
locationN terminal on insert
tagyeast enhanced GFP with 8 amino acids TSGRGEGQ linker
resistance markers :
720
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA