This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLG1-puro-sgULK1-1
catalog :
109004
citations: 1
Reference
Liang J, Lingeman E, Ahmed S, Corn J. Atlastins remodel the endoplasmic reticulum for selective autophagy. J Cell Biol. 2018;217:3354-3367 pubmed publisher
product information
Catalog Number :
109004
Product Name :
pLG1-puro-sgULK1-1
article :
doi10.1083/jcb.201804185
id28193060
pubmed_id30143524
bacterial resistance :
Ampicillin
cloning :
backbonepCRISPRia-v2
backbone_mutation
backbone_originJonathan Weissman, Addgene #84834
backbone_size8200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BlpI
cloning_site_5BstX1
promotermU6 promoter
sequencing_primer_3CCTTCTCTAGGCACCGGTTC
sequencing_primer_5gcgccaattctgcagacaaa
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATG1, ATG1A, UNC51, Unc51.1, hATG1
geneULK1
id8408
genbank_ids
mutation
nameULK1-sgRNA #1
shRNA_sequenceGGCGGCGGCACAGAGACCGT
size
species
9606
Homo sapiens
tags
resistance markers :
2659
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA