This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch
catalog :
10881
citations: 4
Reference
Zhu B, Sun L, Luo W, Li M, Coy D, Yu L, et al. Activated Notch signaling augments cell growth in hepatocellular carcinoma via up-regulating the nuclear receptor NR4A2. Oncotarget. 2017;8:23289-23302 pubmed publisher
Sun L, Liu M, Sun G, Yang X, Qian Q, Feng S, et al. Notch Signaling Activation in Cervical Cancer Cells Induces Cell Growth Arrest with the Involvement of the Nuclear Receptor NR4A2. J Cancer. 2016;7:1388-95 pubmed publisher
Ruegsegger C, Maharjan N, Goswami A, Filézac de L Etang A, Weis J, Troost D, et al. Aberrant association of misfolded SOD1 with Na(+)/K(+)ATPase-α3 impairs its activity and contributes to motor neuron vulnerability in ALS. Acta Neuropathol. 2016;131:427-51 pubmed publisher
Raoul C, Abbas Terki T, Bensadoun J, Guillot S, Haase G, Szulc J, et al. Lentiviral-mediated silencing of SOD1 through RNA interference retards disease onset and progression in a mouse model of ALS. Nat Med. 2005;11:423-8 pubmed
product information
Catalog Number :
10881
Product Name :
pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch
article :
doi10.1038/nm1207
id408
pubmed_id15768028
bacterial resistance :
Ampicillin
cloning :
backbonepCCL-cPPT-PGK-EGFP-WPRE
backbone_mutation
backbone_origin
backbone_size7751
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
Cre/Lox
growth notes :
shSOD1 mismatch sense oligonucleotide: 5'-GATCCCCAAG GATTAATAGA GGCATGTTCA AGAGACATGC CTCTATTAAT CCTTTTTTTG GAAA-3'. Please visit LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.
growth temp :
Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
origin :
37
pi :
alt_names
SOD1
cloning
clone_methodRestriction Enzyme
cloning_site_3ClaI
cloning_site_5ClaI
promoter
sequencing_primer_3
sequencing_primer_5H1 specific primer
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer
geneSOD1
id6647
genbank_ids
mutation
nameH1-shSOD1mismatch
shRNA_sequence
size394
species
9606
Homo sapiens
tags
resistance markers :
6
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA