This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1
catalog :
10880
citations: 4
Reference
Vamva E, Griffiths A, Vink C, Lever A, Kenyon J. A novel role for gag as a cis-acting element regulating RNA structure, dimerization and packaging in HIV-1 lentiviral vectors. Nucleic Acids Res. 2021;: pubmed publisher
Sharif T, Dai C, Martell E, Ghassemi Rad M, Hanes M, Murphy P, et al. TAp73 Modifies Metabolism and Positively Regulates Growth of Cancer Stem-Like Cells in a Redox-Sensitive Manner. Clin Cancer Res. 2019;25:2001-2017 pubmed publisher
Ruegsegger C, Maharjan N, Goswami A, Filézac de L Etang A, Weis J, Troost D, et al. Aberrant association of misfolded SOD1 with Na(+)/K(+)ATPase-α3 impairs its activity and contributes to motor neuron vulnerability in ALS. Acta Neuropathol. 2016;131:427-51 pubmed publisher
Raoul C, Abbas Terki T, Bensadoun J, Guillot S, Haase G, Szulc J, et al. Lentiviral-mediated silencing of SOD1 through RNA interference retards disease onset and progression in a mouse model of ALS. Nat Med. 2005;11:423-8 pubmed
product information
Catalog Number :
10880
Product Name :
pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1
article :
doi10.1038/nm1207
id408
pubmed_id15768028
bacterial resistance :
Ampicillin
cloning :
backbonepCCL-cPPT-PGK-EGFP-WPRE
backbone_mutation
backbone_origin
backbone_size7751
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
Cre/Lox
growth notes :
shSOD1 sense oligonucleotide: 5'-GATCCCCAAG GATGAAGAGA GGCATGTTCA AGAGACATGC CTCTCTTCAT CCTTTTTTTG GAAA-3'. Please visit LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.
growth temp :
Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
origin :
37
pi :
alt_names
shSOD1
SOD1
cloning
clone_methodRestriction Enzyme
cloning_site_3ClaI
cloning_site_5ClaI
promoter
sequencing_primer_3
sequencing_primer_5H1 specific primer
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer
geneSOD1
id6647
genbank_ids
mutation
nameH1-shSOD1
shRNA_sequence
size394
species
9606
Homo sapiens
tags
resistance markers :
6
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA