pGEX6P1 hsRNASEH2BCA(D34A/D169A) | Addgene 108693 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGEX6P1 hsRNASEH2BCA(D34A/D169A)

catalog :

108693

citations: 2

Reference
Alecki C, Francis N. Identification of R-loop-forming Sequences in Drosophila melanogaster Embryos and Tissue Culture Cells Using DRIP-seq. Bio Protoc. 2021;11:e4011 pubmed publisher
Reijns M, Bubeck D, Gibson L, Graham S, Baillie G, Jones E, et al. The structure of the human RNase H2 complex defines key interaction interfaces relevant to enzyme function and human disease. J Biol Chem. 2011;286:10530-9 pubmed publisher

product information

Catalog Number :

108693

Product Name :

pGEX6P1 hsRNASEH2BCA(D34A/D169A)

article :

doi	10.1074/jbc.M110.177394
id	28193014
pubmed_id	21177854

bacterial resistance :

Ampicillin

cloning :

backbone

pGEX6P1

backbone_mutation

SacI site added between between RNASEH2A stop codon and NotI site

backbone_origin

Amersham

backbone_size

4984

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	ClaI
cloning_site_5	SalI
promoter	tac
sequencing_primer_3	CGTCGCCGCTCTCCATATG
sequencing_primer_5	GGGCTGGCAAGCCACGTTTGGTG
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

excluding methionine start

name

RNASEH2B

shRNA_sequence

size

938

species

9606

Homo sapiens