pMSCVpuro-eGFP-mcGAS | Addgene 108675 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMSCVpuro-eGFP-mcGAS

catalog :

108675

citations: 4

Reference
Liu J, Bu X, Chu C, Dai X, Asara J, Sicinski P, et al. PRMT1 mediated methylation of cGAS suppresses anti-tumor immunity. Nat Commun. 2023;14:2806 pubmed publisher
Haarer E, Theodore C, Guo S, Frier R, Campellone K. Genomic instability caused by Arp2/3 complex inactivation results in micronucleus biogenesis and cellular senescence. PLoS Genet. 2023;19:e1010045 pubmed publisher
Ryabchenko B, Soldatova I, Sroller V, Forstova J, Huerfano S. Immune sensing of mouse polyomavirus DNA by p204 and cGAS DNA sensors. FEBS J. 2021;288:5964-5985 pubmed publisher
Mackenzie K, Carroll P, Martin C, Murina O, Fluteau A, Simpson D, et al. cGAS surveillance of micronuclei links genome instability to innate immunity. Nature. 2017;548:461-465 pubmed publisher

product information

Catalog Number :

108675

Product Name :

pMSCVpuro-eGFP-mcGAS

article :

doi	10.1038/nature23449
id	28193007
pubmed_id	28738408

bacterial resistance :

Ampicillin

cloning :

backbone

pMSCVpuro

backbone_mutation

backbone_origin

Clontech

backbone_size

6264

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Retroviral

growth strain :

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged mouse cGAS

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	HpaI
cloning_site_5	HpaI
promoter	LTR
sequencing_primer_3	GAGACGTGCTACTTCCATTTGTC
sequencing_primer_5	CCCTTGAACCTCCTCGTTCGACC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	E330016A19Rik, Mb21d1
gene	Cgas
id	214763

genbank_ids

mutation

name

eGFP-cGAS

shRNA_sequence

size

2340

species

10090

Mus musculus

32644

Other