This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCR3-Flag-AuroraB KD (K106R)
catalog :
108488
citations: 1
Reference
Hengeveld R, Hertz N, Vromans M, Zhang C, Burlingame A, Shokat K, et al. Development of a chemical genetic approach for human aurora B kinase identifies novel substrates of the chromosomal passenger complex. Mol Cell Proteomics. 2012;11:47-59 pubmed publisher
product information
Catalog Number :
108488
Product Name :
pCR3-Flag-AuroraB KD (K106R)
article :
doi10.1074/mcp.M111.013912
id28192946
pubmed_id22267324
bacterial resistance :
Ampicillin
cloning :
backbonepCR3
backbone_mutation
backbone_origin
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Addgene's sequencing results found M298T in AuroraB compared to the NCBI reference. The depositing lab states that there is a SNP at this location, and the discrepancy should not affect plasmid function.
growth strain :
expression of Flag-AuroraB kinase death (K106R)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR1
cloning_site_5EcoR1
promoterCMV
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAIK2, AIM-1, AIM1, ARK-2, ARK2, AurB, IPL1, PPP1R48, STK-1, STK12, STK5, aurkb-sv1, aurkb-sv2
geneAURKB
id9212
genbank_ids
mutationKinase death K106A
nameAuroraB
shRNA_sequence
size1035
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
resistance markers :
1658
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA