This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
myrAkt delta4-129
catalog :
10841
citations: 30
Reference
Mahmud J, Geiler B, Biswas J, Miller M, Myers J, Matthews S, et al. Virion-associated US28 rapidly modulates Akt activity to suppress HCMV lytic replication in monocytes. bioRxiv. 2023;: pubmed publisher
Li K, Sun X, Minami K, Tamari K, Ogawa K, Li H, et al. Proteomes from AMPK-inhibited peripheral blood mononuclear cells suppress the progression of breast cancer and bone metastasis. Theranostics. 2023;13:1247-1263 pubmed publisher
Patel H, Mishra R, Wier A, Mokhtarpour N, Merino E, Garrett J. RIDR-PI-103, ROS-activated prodrug PI3K inhibitor inhibits cell growth and impairs the PI3K/Akt pathway in BRAF and MEK inhibitor-resistant BRAF-mutant melanoma cells. Anticancer Drugs. 2023;34:519-531 pubmed publisher
Urushima H, Matsubara T, Miyakoshi M, Kimura S, Yuasa H, Yoshizato K, et al. Hypo-osmolarity induces apoptosis resistance via TRPV2-mediated AKT-Bcl-2 pathway. Am J Physiol Gastrointest Liver Physiol. 2023;324:G219-G230 pubmed publisher
Kim H, Huh Y, Kim J, Jo M, Shin J, Park S, et al. Identification and evaluation of midbrain specific longevity-related genes in exceptionally long-lived but healthy mice. Front Aging Neurosci. 2022;14:1030807 pubmed publisher
Sun X, Li K, Aryal U, Li B, Yokota H. PI3K-activated MSC proteomes inhibit mammary tumors via Hsp90ab1 and Myh9. Mol Ther Oncolytics. 2022;26:360-371 pubmed publisher
Zhang H, Domma A, Goodrum F, Moorman N, Kamil J. The Akt Forkhead Box O Transcription Factor Axis Regulates Human Cytomegalovirus Replication. MBio. 2022;13:e0104222 pubmed publisher
Feng L, Feng S, Nie Z, Deng Y, Xuan Y, Chen X, et al. TRAF6 Promoted Tumor Glycolysis in Non-Small-Cell Lung Cancer by Activating the Akt-HIFα Pathway. Biomed Res Int. 2021;2021:3431245 pubmed publisher
Sun X, Li K, Zha R, Liu S, Fan Y, Wu D, et al. Preventing tumor progression to the bone by induced tumor-suppressing MSCs. Theranostics. 2021;11:5143-5159 pubmed publisher
Perfetto M, Xu X, Lu C, Shi Y, Yousaf N, Li J, et al. The RNA helicase DDX3 induces neural crest by promoting AKT activity. Development. 2021;148: pubmed publisher
Zhao H, Chen G, Liang H. Dual PI3K/mTOR Inhibitor, XL765, suppresses glioblastoma growth by inducing ER stress-dependent apoptosis. Onco Targets Ther. 2019;12:5415-5424 pubmed publisher
Wani A, Gupta M, Ahmad M, Shah A, Ahsan A, Qazi P, et al. Alborixin clears amyloid-β by inducing autophagy through PTEN-mediated inhibition of the AKT pathway. Autophagy. 2019;:1-19 pubmed publisher
Yap H, Lee Y, Harith H, Tham C, Cheema M, Shaari K, et al. The geranyl acetophenone tHGA attenuates human bronchial smooth muscle proliferation via inhibition of AKT phosphorylation. Sci Rep. 2018;8:16640 pubmed publisher
Balboa D, Weltner J, Novik Y, Eurola S, Wartiovaara K, Otonkoski T. Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9. Stem Cell Res. 2017;23:105-108 pubmed publisher
Wang H, Wang Y, Wu D, Hung C, Chen C, Lee H. Targeting insulin-like growth factor-binding protein-3 by microRNA-125b promotes tumor invasion and poor outcomes in non-small-cell lung cancer. Tumour Biol. 2017;39:1010428317694316 pubmed publisher
Li L, Chen J, Xiong G, St Clair D, Xu W, Xu R. Increased ROS production in non-polarized mammary epithelial cells induces monocyte infiltration in 3D culture. J Cell Sci. 2017;130:190-202 pubmed publisher
Li Y, Chandrasekaran V, Carter S, Woodward C, Christensen D, Dryden K, et al. Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. elife. 2016;5: pubmed publisher
Vallath S, Sage E, Kolluri K, Lourenco S, Teixeira V, Chimalapati S, et al. CADM1 inhibits squamous cell carcinoma progression by reducing STAT3 activity. Sci Rep. 2016;6:24006 pubmed publisher
Guo X, Snider W, Chen B. GSK3β regulates AKT-induced central nervous system axon regeneration via an eIF2Bε-dependent, mTORC1-independent pathway. elife. 2016;5:e11903 pubmed publisher
Caropreso V, Darvishi E, Turbyville T, Ratnayake R, Grohar P, McMahon J, et al. Englerin A Inhibits EWS-FLI1 DNA Binding in Ewing Sarcoma Cells. J Biol Chem. 2016;291:10058-66 pubmed publisher
Zhang Y, Stefanovic B. Akt mediated phosphorylation of LARP6; critical step in biosynthesis of type I collagen. Sci Rep. 2016;6:22597 pubmed publisher
Cui Y, Jiao H, Ye Y, Chen C, Wang J, Tang N, et al. FOXC2 promotes colorectal cancer metastasis by directly targeting MET. Oncogene. 2015;34:4379-90 pubmed publisher
Zhu L, Derijard B, Chakrabandhu K, Wang B, Chen H, Hueber A. Synergism of PI3K/Akt inhibition and Fas activation on colon cancer cell death. Cancer Lett. 2014;354:355-64 pubmed publisher
Sherwood J, Bertrand J, Nalesso G, Poulet B, Pitsillides A, Brandolini L, et al. A homeostatic function of CXCR2 signalling in articular cartilage. Ann Rheum Dis. 2015;74:2207-15 pubmed publisher
Weissinger D, Tagscherer K, Macher Göppinger S, Haferkamp A, Wagener N, Roth W. The soluble Decoy Receptor 3 is regulated by a PI3K-dependent mechanism and promotes migration and invasion in renal cell carcinoma. Mol Cancer. 2013;12:120 pubmed publisher
Meyer R, D Alessandro L, Kar S, Kramer B, She B, Kaschek D, et al. Heterogeneous kinetics of AKT signaling in individual cells are accounted for by variable protein concentration. Front Physiol. 2012;3:451 pubmed publisher
Chu C, Chen C, Rajendran R, Shen C, Chen T, Yen C, et al. Overexpression of Akt1 enhances adipogenesis and leads to lipoma formation in zebrafish. PLoS ONE. 2012;7:e36474 pubmed publisher
Bhoopathi P, Gorantla B, Sailaja G, Gondi C, Gujrati M, Klopfenstein J, et al. SPARC overexpression inhibits cell proliferation in neuroblastoma and is partly mediated by tumor suppressor protein PTEN and AKT. PLoS ONE. 2012;7:e36093 pubmed publisher
Rao V, Kandel A, Lynch D, Pena Z, Marwaha N, Deng C, et al. A positive feedback loop between HER2 and ADAM12 in human head and neck cancer cells increases migration and invasion. Oncogene. 2012;31:2888-98 pubmed publisher
Kohn A, Takeuchi F, Roth R. Akt, a pleckstrin homology domain containing kinase, is activated primarily by phosphorylation. J Biol Chem. 1996;271:21920-6 pubmed
product information
Catalog Number :
10841
Product Name :
myrAkt delta4-129
article :
doi10.1074/jbc.271.36.21920
id390
pubmed_id8702995
bacterial resistance :
Ampicillin
cloning :
backbonepECE
backbone_mutation
backbone_originWilliam Rutter
backbone_size2924
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Constitutively active Akt. 14 aa src myristoylation signal sequence fused to N terminus of Akt delta4-129. Note: Addgene NGS results detected an 8 bp deletion in the 5' end of the SV40 promoter. Plasmid should function as described in the associated publication.
origin :
37
pi :
alt_names
myr Akt deltaPH
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5unknown
promoterSV40
sequencing_primer_3SV40pA-R
sequencing_primer_5SV40pro-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
geneAKT1
id207
genbank_ids
mutationDeletion of PH domain (aa4-129)
nameAkt
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmyr
locationC terminal on insert
tagHA
resistance markers :
426
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA