This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3 flag p53
catalog :
10838
citations: 46
Reference
Mansky R, Greguske E, Yu D, Zarate N, Intihar T, Tsai W, et al. Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease. Cell Rep. 2023;42:112198 pubmed publisher
Valente J, Carreira T, Dias J, Sousa F, Alves N. Arginine-Modified 3D-Printed Chromatographic Supports. Pharmaceutics. 2022;14: pubmed publisher
Neves A, Albuquerque T, Faria R, Gon xe7 alves A, Santos C, Viv xe8 s E, et al. Development of WRAP5 Peptide Complexes for Targeted Drug/Gene Co-Delivery toward Glioblastoma Therapy. Pharmaceutics. 2022;14: pubmed publisher
Galhuber M, Michenthaler H, Heininger C, Reinisch I, Nössing C, Krstić J, et al. Complementary omics strategies to dissect p53 signaling networks under nutrient stress. Cell Mol Life Sci. 2022;79:326 pubmed publisher
Albuquerque T, Neves A, Quintela T, Costa D. The Influence of Circadian Rhythm on Cancer Cells Targeting and Transfection Efficiency of a Polycation-Drug/Gene Delivery Vector. Polymers (Basel). 2022;14: pubmed publisher
Neves A, Albuquerque T, Faria R, Paul M, Biswas S, Sousa A, et al. Development of Tailor-Made Dendrimer Ternary Complexes for Drug/Gene Co-Delivery in Cancer. Pharmaceutics. 2021;13: pubmed publisher
Valente J, Pereira P, Sousa A, Queiroz J, Sousa F. Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation. Polymers (Basel). 2021;13: pubmed publisher
Sheikh T, Sen E. p53 affects epigenetic signature on SOCS1 promoter in response to TLR4 inhibition. Cytokine. 2021;140:155418 pubmed publisher
Neves M, Pereira P, Pedro A, Martins J, Trindade T, Queiroz J, et al. Improved ionic-liquid-functionalized macroporous supports able to purify nucleic acids in one step. Mater Today Bio. 2020;8:100086 pubmed publisher
Passarinha L. Enhanced Biosynthesis of Plasmid DNA from Escherichia coli Applying Experimental Design. Methods Mol Biol. 2021;2197:135-150 pubmed publisher
Gerakopoulos V, Ngo P, Tsiokas L. Loss of polycystins suppresses deciliation via the activation of the centrosomal integrity pathway. Life Sci Alliance. 2020;3: pubmed publisher
Valente J, Sousa A, Azevedo G, Queiroz J, Sousa F. Purification of supercoiled p53-encoding plasmid using an arginine-modified macroporous support. J Chromatogr A. 2020;1618:460890 pubmed publisher
Arisan E, Akar R, Rencüzoğulları Ö, Obakan Yerlikaya P, Coker Gurkan A, Akın B, et al. The molecular targets of diclofenac differs from ibuprofen to induce apoptosis and epithelial mesenchymal transition due to alternation on oxidative stress management p53 independently in PC3 prostate cancer cells. Prostate Int. 2019;7:156-165 pubmed publisher
Kırımtay K, Selçuk E, Kelle D, Erman B, Karabay A. p53 regulates katanin-p60 promoter in HCT 116 cells. Gene. 2020;727:144241 pubmed publisher
Neves A, Sousa A, Faria R, Albuquerque T, Queiroz J, Costa D. Cancer gene therapy mediated by RALA/plasmid DNA vectors: Nitrogen to phosphate groups ratio (N/P) as a tool for tunable transfection efficiency and apoptosis. Colloids Surf B Biointerfaces. 2020;185:110610 pubmed publisher
Sousa A, Almeida A, Faria R, Konate K, Boisguerin P, Queiroz J, et al. Optimization of peptide-plasmid DNA vectors formulation for gene delivery in cancer therapy exploring design of experiments. Colloids Surf B Biointerfaces. 2019;183:110417 pubmed publisher
Valente J, Sousa A, Queiroz J, Sousa F. DoE to improve supercoiled p53-pDNA purification by O-phospho-l-tyrosine chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2019;1105:184-192 pubmed publisher
Zella D, Curreli S, Benedetti F, Krishnan S, Cocchi F, Latinovic O, et al. Mycoplasma promotes malignant transformation in vivo, and its DnaK, a bacterial chaperon protein, has broad oncogenic properties. Proc Natl Acad Sci U S A. 2018;115:E12005-E12014 pubmed publisher
Chen G, Zhou T, Liu Y, Yu Z. Combinatory inhibition of TRIM65 and MDM2 in lung cancer cells. Biochem Biophys Res Commun. 2018;506:698-702 pubmed publisher
Chen H, Xue L, Huang H, Wang H, Zhang X, Zhu W, et al. Synergistic effect of Nutlin-3 combined with MG-132 on schwannoma cells through restoration of merlin and p53 tumour suppressors. EBioMedicine. 2018;36:252-265 pubmed publisher
Costa D, Valente A, Queiroz J, Sousa A. Finding the ideal polyethylenimine-plasmid DNA system for co-delivery of payloads in cancer therapy. Colloids Surf B Biointerfaces. 2018;170:627-636 pubmed publisher
Xie X, Wang X, Liao W, Fei R, Wu N, Cong X, et al. MYL6B, a myosin light chain, promotes MDM2-mediated p53 degradation and drives HCC development. J Exp Clin Cancer Res. 2018;37:28 pubmed publisher
Ullah K, Rosendahl A, Izzi V, Bergmann U, Pihlajaniemi T, Maki J, et al. Hypoxia-inducible factor prolyl-4-hydroxylase-1 is a convergent point in the reciprocal negative regulation of NF-κB and p53 signaling pathways. Sci Rep. 2017;7:17220 pubmed publisher
Costa D, Costa C, Caldeira M, Cortes L, Queiroz J, Cruz C. Targeting of Cellular Organelles by Fluorescent Plasmid DNA Nanoparticles. Biomacromolecules. 2017;18:2928-2936 pubmed publisher
Nguyen D, Richter D, Michel G, Mitschka S, Kolanus W, Cuevas E, et al. The ubiquitin ligase LIN41/TRIM71 targets p53 to antagonize cell death and differentiation pathways during stem cell differentiation. Cell Death Differ. 2017;24:1063-1078 pubmed publisher
Chen G, Zhou T, Li Y, Yu Z, Sun L. p53 target miR-29c-3p suppresses colon cancer cell invasion and migration through inhibition of PHLDB2. Biochem Biophys Res Commun. 2017;487:90-95 pubmed publisher
Zhou X, Shi H, Yang S, Sun P. An efficient rapid system for assaying HBx-mediated transactivation. Biotechnol Lett. 2017;39:1091-1099 pubmed publisher
Xu W, Li B, Guan X, Chung S, Wang Y, Yip Y, et al. Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression. Nat Commun. 2017;8:14399 pubmed publisher
Mattison S, Blatch G, Edkins A. HOP expression is regulated by p53 and RAS and characteristic of a cancer gene signature. Cell Stress Chaperones. 2017;22:213-223 pubmed publisher
Zhai N, Xia Y, Yin R, Liu J, Gao F. A negative regulation loop of long noncoding RNA HOTAIR and p53 in non-small-cell lung cancer. Onco Targets Ther. 2016;9:5713-5720 pubmed
Schmitt A, Garcia J, Hung T, Flynn R, Shen Y, Qu K, et al. An inducible long noncoding RNA amplifies DNA damage signaling. Nat Genet. 2016;48:1370-1376 pubmed publisher
Li Y, Ma C, Zhou T, Liu Y, Sun L, Yu Z. TRIM65 negatively regulates p53 through ubiquitination. Biochem Biophys Res Commun. 2016;473:278-282 pubmed publisher
Barroso González J, Auclair S, Luan S, Thomas L, Atkins K, Aslan J, et al. PACS-2 mediates the ATM and NF-κB-dependent induction of anti-apoptotic Bcl-xL in response to DNA damage. Cell Death Differ. 2016;23:1448-57 pubmed publisher
Laezza C, D Alessandro A, Di Croce L, Picardi P, Ciaglia E, Pisanti S, et al. p53 regulates the mevalonate pathway in human glioblastoma multiforme. Cell Death Dis. 2015;6:e1909 pubmed publisher
Bock F, Tanzer M, Haschka M, Krumschnabel G, Sohm B, Goetsch K, et al. The p53 binding protein PDCD5 is not rate-limiting in DNA damage induced cell death. Sci Rep. 2015;5:11268 pubmed publisher
Richter M, Dayaram T, Gilmartin A, Ganji G, Pemmasani S, Van Der Key H, et al. WIP1 phosphatase as a potential therapeutic target in neuroblastoma. PLoS ONE. 2015;10:e0115635 pubmed publisher
Costa D, Valente A, Queiroz J. Plasmid DNA nanogels as photoresponsive materials for multifunctional bio-applications. J Biotechnol. 2015;202:98-104 pubmed publisher
Obakan P, Arisan E, Coker Gurkan A, Palavan Unsal N. Epibrassinolide-induced apoptosis regardless of p53 expression via activating polyamine catabolic machinery, a common target for androgen sensitive and insensitive prostate cancer cells. Prostate. 2014;74:1622-33 pubmed publisher
Santos J, Sousa F, Queiroz J, Costa D. Rhodamine based plasmid DNA nanoparticles for mitochondrial gene therapy. Colloids Surf B Biointerfaces. 2014;121:129-40 pubmed publisher
Valente J, Sousa A, Queiroz J, Sousa F. Selective purification of supercoiled p53-encoding pDNA with L-methionine-agarose matrix. Anal Biochem. 2014;459:61-9 pubmed publisher
Feldman D, Chen C, Punj V, Machida K. The TBC1D15 oncoprotein controls stem cell self-renewal through destabilization of the Numb-p53 complex. PLoS ONE. 2013;8:e57312 pubmed publisher
Bertin Ciftci J, Barre B, Le Pen J, Maillet L, Couriaud C, Juin P, et al. pRb/E2F-1-mediated caspase-dependent induction of Noxa amplifies the apoptotic effects of the Bcl-2/Bcl-xL inhibitor ABT-737. Cell Death Differ. 2013;20:755-64 pubmed publisher
Heo K, Lee H, Nigro P, Thomas T, Le N, Chang E, et al. PKC? mediates disturbed flow-induced endothelial apoptosis via p53 SUMOylation. J Cell Biol. 2011;193:867-84 pubmed publisher
Wu L, Ma C, Zhao Y, Jain A. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression. J Biol Chem. 2011;286:2236-44 pubmed publisher
Chugh P, Bradel Tretheway B, Monteiro Filho C, Planelles V, Maggirwar S, Dewhurst S, et al. Akt inhibitors as an HIV-1 infected macrophage-specific anti-viral therapy. Retrovirology. 2008;5:11 pubmed publisher
Gjoerup O, Zaveri D, Roberts T. Induction of p53-independent apoptosis by simian virus 40 small t antigen. J Virol. 2001;75:9142-55 pubmed
product information
Catalog Number :
10838
Product Name :
pcDNA3 flag p53
article :
doi10.1128/JVI.75.19.9142-9155.2001
id388
pubmed_id11533178
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5BamHI
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutation
namep53
shRNA_sequence
size1200
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagflag
resistance markers :
419
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA