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product summary
company name :
Addgene
product type :
cDNA
product name :
pLNCX Myr His p110 KD
catalog :
10837
citations: 2
| Reference |
|---|
Auger K, Wang J, Narsimhan R, Holcombe T, Roberts T. Constitutive cellular expression of PI 3-kinase is distinct from transient expression. Biochem Biophys Res Commun. 2000;272:822-9 pubmed
|
product information
Catalog Number :
10837
Product Name :
pLNCX Myr His p110 KD
article :
| doi | 10.1006/bbrc.2000.2806 |
| id | 375 |
| pubmed_id | 10860837 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pLNCX | ||
| backbone_mutation | |||
| backbone_origin | BD Biosciences | ||
| backbone_size | 6620 | ||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
A pair of overlapping oligomers were synthesized based on the c-src myristoylation signal peptide sequence. A Kozak sequence was engineered at the 5 of the c-src myristoylation signal sequence (MetGly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Asp-Pro-Ser-Gln-Arg-Arg-ArgArg-Ile-Arg-Thr). The fragment was ligated to the 5 end of bovine p110 sequence and cloned into a retroviral expression vector (pLNCX) by a three piece ligation reaction. A 6 histidine tag was incorporated on the 3 end of p110 by PCR. The kinase dead mutant has an additional BamHI site at D915S.
origin :
37
pi :
|
resistance markers :
| 419 |
tags :
High Copy
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
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