Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605 | Addgene 108362 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

catalog :

108362

citations: 1

Reference
Alberti C, Manzenreither R, Sowemimo I, Burkard T, Wang J, Mahofsky K, et al. Cell-type specific sequencing of microRNAs from complex animal tissues. Nat Methods. 2018;15:283-289 pubmed publisher

product information

Catalog Number :

108362

Product Name :

Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

article :

doi	10.1038/nmeth.4610
id	28192930
pubmed_id	29481550

bacterial resistance :

Ampicillin

cloning :

backbone

pCFJ350-ttTi5605

backbone_mutation

The cassette for bicistronic expression of Ath-HEN1 and mCherry-H2B was inserted into pCFJ350-ttTi5605 by Gibson cloning between the M13 FWD and M13 REV priming sites.

backbone_origin

backbone_size

7517

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Worm Expression

growth strain :

Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans

origin :

pi :

CORYMBOSA 2, CRM2, HUA ENHANCER 1, T13K14.70, T13K14_70geneHEN1id827839genbank_idsmutationnameAth-HEN1shRNA_sequencesize2979species

3702

Arabidopsis thaliana

32644

Other

Codon optimised for C. elegans

tags

location	N terminal on insert
tag	2xFlag

location	N terminal on insert
tag	MYC

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	mCherry
sequencing_primer_3	TCCTGATTGGTATGCACTTCcttgtacagctcgtccatgcc
sequencing_primer_5	AGAAGAAGAAGACCGACCTCgagggcagaggaagtctgc
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

mCherry

shRNA_sequence

size

708

species

32644

Other

tags

location	N terminal on insert
tag	T2A peptide

alt_names

histone sequence

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	H2B
sequencing_primer_3	CTCAGATATCAATACCATGCCTATTACTTGCTGGAAGTGTACTTGG
sequencing_primer_5	GAAGTGCATACCAATCAGGACCC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CELE_ZK131.9
gene	his-15
id	175026

genbank_ids

mutation

name

his-15

shRNA_sequence

size

369

species

6239

Caenorhabditis elegans

tags

location	N terminal on insert
tag	TY1 linker

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	AvrII
cloning_site_5	AvrII
promoter	A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as a unique AvrII fragment from an intermediate vector into pCFJ350-ttTi5605
sequencing_primer_3	AAAACCTAGGCACCGTCATCACCGAAACGC
sequencing_primer_5	TTTTCCTAGGGATTACGCCAAGCTTGCATGC
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

unc-54 3' UTR

shRNA_sequence

size

733

species

6239

Caenorhabditis elegans

tags

sequencesequencing_pr
imer_3GAGGTCGGTCTTCTTCTTCTCAAC
sequencing_primer_5td>CCGGCCggcgcgccGCTAGCcaccatggatta
caaggatgacgsite_3_des
troyedsite_5
_destroyedtd>entrez_geneble>

aliases

AT4G20910,

alt_names

A. thaliana HEN1

cloning

clone_method

Gibson Cloning

cloning_site_3

cloning_site_5

promoter

Ath-HEN1 coding

resistance markers :

3840

tags :

High Copy