pLNCX Myr His p110 | Addgene 10836 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLNCX Myr His p110

catalog :

10836

citations: 1

Reference
Auger K, Wang J, Narsimhan R, Holcombe T, Roberts T. Constitutive cellular expression of PI 3-kinase is distinct from transient expression. Biochem Biophys Res Commun. 2000;272:822-9 pubmed

product information

Catalog Number :

10836

Product Name :

pLNCX Myr His p110

article :

doi	10.1006/bbrc.2000.2806
id	375
pubmed_id	10860837

bacterial resistance :

Ampicillin

cloning :

backbone

pLNCX

backbone_mutation

backbone_origin

BD Biosciences

backbone_size

6620

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

A pair of overlapping oligomers were synthesized based on the c-src myristoylation signal peptide sequence. A Kozak sequence was engineered at the 5 of the c-src myristoylation signal sequence (MetGly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Asp-Pro-Ser-Gln-Arg-Arg-ArgArg-Ile-Arg-Thr). The fragment was ligated to the 5 end of bovine p110 sequence and cloned into a retroviral expression vector (pLNCX) by a three piece ligation reaction. A 6 histidine tag was incorporated on the 3 end of p110 by PCR.

origin :

pi :

alt_names

PI3 Kinase

PI3K

PIK3CA

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	HindIII
promoter
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	PIK3CA
id	282306

genbank_ids

NM_174574

mutation

name

p110

shRNA_sequence

size

species

9913

Bos taurus