This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pIRESneo-FLAG/HA Ago2
catalog :
10821
citations: 3
Reference
Hoang V, Matossian M, Ucar D, Elliott S, La J, Wright M, et al. ERK5 Is Required for Tumor Growth and Maintenance Through Regulation of the Extracellular Matrix in Triple Negative Breast Cancer. Front Oncol. 2020;10:1164 pubmed publisher
Conger A, Martin E, Yan T, Rhodes L, Hoang V, La J, et al. Argonaute 2 Expression Correlates with a Luminal B Breast Cancer Subtype and Induces Estrogen Receptor Alpha Isoform Variation. Noncoding RNA. 2016;2: pubmed publisher
Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Mol Cell. 2004;15:185-97 pubmed
product information
Catalog Number :
10821
Product Name :
pIRESneo-FLAG/HA Ago2
article :
doi10.1016/j.molcel.2004.07.007
id384
pubmed_id15260970
bacterial resistance :
Ampicillin
cloning :
backbonepIRESneo
backbone_mutation
backbone_originClontech
backbone_size5313
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
A small insertion in the 5'UTR should not affect the expressed peptide.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5NotI
promoter
sequencing_primer_3agctgttggggtgagtactcc
sequencing_primer_5ggtaccgagctcggatcgat
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10
geneAGO2
id27161
genbank_ids
NM_012154
mutationR668H. Additionally, N terminus different from wild-type - Initial 18 nucleotides of the wild-type sequence were replaced with alternate 21 nucleotides. No difference in activity observed.
nameArgonaute 2
shRNA_sequence
size2580
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG
locationN terminal on insert
tagHA
resistance markers :
536
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA