This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pIRESneo-FLAG/HA Ago1
catalog :
10820
citations: 15
Reference
Shen Q, Wang Y, Truong M, Mahadevan K, Wu J, Zhang H, et al. RanBP2/Nup358 enhances miRNA activity by sumoylating Argonautes. PLoS Genet. 2021;17:e1009378 pubmed publisher
Shin E, Jin H, Suh D, Luo Y, Ha H, Kim T, et al. An alternative miRISC targets a cancer-associated coding sequence mutation in FOXL2. EMBO J. 2020;:e104719 pubmed publisher
Adiliaghdam F, Basavappa M, Saunders T, Harjanto D, Prior J, Cronkite D, et al. A Requirement for Argonaute 4 in Mammalian Antiviral Defense. Cell Rep. 2020;30:1690-1701.e4 pubmed publisher
Sheu Gruttadauria J, Pawlica P, Klum S, Wang S, Yario T, Schirle Oakdale N, et al. Structural Basis for Target-Directed MicroRNA Degradation. Mol Cell. 2019;75:1243-1255.e7 pubmed publisher
Sun G, Wang J, Huang Y, Yuan C, Zhang K, Hu S, et al. Differences in silencing of mismatched targets by sliced versus diced siRNAs. Nucleic Acids Res. 2018;46:6806-6822 pubmed publisher
Harwig A, Kruize Z, Yang Z, Restle T, Berkhout B. Analysis of AgoshRNA maturation and loading into Ago2. PLoS ONE. 2017;12:e0183269 pubmed publisher
Golden R, Chen B, Li T, Braun J, Manjunath H, Chen X, et al. An Argonaute phosphorylation cycle promotes microRNA-mediated silencing. Nature. 2017;542:197-202 pubmed publisher
Müller C, Schäfer I, Luhmann H, White R. Oligodendroglial Argonaute protein Ago2 associates with molecules of the Mbp mRNA localization machinery and is a downstream target of Fyn kinase. Front Cell Neurosci. 2015;9:328 pubmed publisher
Roy Chaudhuri B, Valdmanis P, Zhang Y, Wang Q, Luo Q, Kay M. Regulation of microRNA-mediated gene silencing by microRNA precursors. Nat Struct Mol Biol. 2014;21:825-32 pubmed publisher
Huang V, Zheng J, Qi Z, Wang J, Place R, Yu J, et al. Ago1 Interacts with RNA polymerase II and binds to the promoters of actively transcribed genes in human cancer cells. PLoS Genet. 2013;9:e1003821 pubmed publisher
Martinez N, Chang H, Borrajo J, Gregory R. The co-chaperones Fkbp4/5 control Argonaute2 expression and facilitate RISC assembly. RNA. 2013;19:1583-93 pubmed publisher
Chen Y, Yuan R, Yang W, Hsu H, Jeng Y. The stem cell E3-ligase Lin-41 promotes liver cancer progression through inhibition of microRNA-mediated gene silencing. J Pathol. 2013;229:486-96 pubmed publisher
Chu Y, Yue X, Younger S, Janowski B, Corey D. Involvement of argonaute proteins in gene silencing and activation by RNAs complementary to a non-coding transcript at the progesterone receptor promoter. Nucleic Acids Res. 2010;38:7736-48 pubmed publisher
Buchet Poyau K, Courchet J, Le Hir H, Seraphin B, Scoazec J, Duret L, et al. Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies. Nucleic Acids Res. 2007;35:1289-300 pubmed
Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Mol Cell. 2004;15:185-97 pubmed
product information
Catalog Number :
10820
Product Name :
pIRESneo-FLAG/HA Ago1
article :
doi10.1016/j.molcel.2004.07.007
id384
pubmed_id15260970
bacterial resistance :
Ampicillin
cloning :
backbonepIRESneo
backbone_mutation
backbone_originClontech
backbone_size5313
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
A small insertion in the 5'UTR should not affect the expressed peptide.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5NotI
promoter
sequencing_primer_3agctgttggggtgagtactcc
sequencing_primer_5ggtaccgagctcggatcgat
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesEIF2C, EIF2C1, GERP95, NEDLBAS, Q99, hAgo1
geneAGO1
id26523
genbank_ids
NM_012199
mutation
nameArgonaute 1
shRNA_sequence
size2571
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG
locationN terminal on insert
tagHA
resistance markers :
536
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA