This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 dCTD EcoRV)
catalog :
107860
product information
Catalog Number :
107860
Product Name :
pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 dCTD EcoRV)
article :
doi
id28192855
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepBI
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Cut with EcoRV (61bp spacer is lost) and use Gibson Cloning to insert CTD variant. Remove EcoRV during Gibson reaction to prevent unwanted aa insertion.
growth strain :
To quickly insert CTD mutants into TDP43 splicing reporter
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALS10, TDP-43
geneTARDBP
id23435
genbank_ids
mutationDeletion of CTD in TDP43 splicing reporter, insertion of EcoRV site
nameTARDBP mutant
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
1010
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA