This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-hSynapsin-FLEX-soCoChR-GFP
catalog :
107712
citations: 4
Reference
Reed F, Reichenbach A, Dempsey H, Clarke R, Méquinion M, Stark R, et al. Acute inhibition of hunger-sensing AgRP neurons promotes context-specific learning in mice. Mol Metab. 2023;77:101803 pubmed publisher
Hagihara K, Bukalo O, Zeller M, Aksoy Aksel A, Karalis N, Limoges A, et al. Intercalated amygdala clusters orchestrate a switch in fear state. Nature. 2021;594:403-407 pubmed publisher
Forli A, Pisoni M, Printz Y, Yizhar O, Fellin T. Optogenetic strategies for high-efficiency all-optical interrogation using blue-light-sensitive opsins. elife. 2021;10: pubmed publisher
Shemesh O, Tanese D, Zampini V, Linghu C, Piatkevich K, Ronzitti E, et al. Temporally precise single-cell-resolution optogenetics. Nat Neurosci. 2017;20:1796-1806 pubmed publisher
product information
Catalog Number :
107712
Product Name :
pAAV-hSynapsin-FLEX-soCoChR-GFP
article :
doi10.1038/s41593-017-0018-8
id28192820
pubmed_id29184208
bacterial resistance :
Ampicillin
cloning :
backbonepAAV
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
growth notes :
GenBank IDs: soCoChR = MF795583, KA2(1-150) = MF795584
growth strain :
A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent virus
origin :
37
pi :
alt_names
CoChR-ggsggtggsggt-KA2-(1-150)
Chloromonas oogama channelrhodopsin, fused to KA2 targeting sequence
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterhSynapsin
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namesoCoChR-GFP
shRNA_sequence
size2013
species
32644
Other
Chloromonas oogama
tags
locationC terminal on insert
tagKA2
locationC terminal on insert
tagEGFP
resistance markers :
50
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA