pAAV-AICD-NES-IRES-hrGFP | Addgene 107544 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAAV-AICD-NES-IRES-hrGFP

catalog :

107544

citations: 1

Reference
Pousinha P, Mouska X, Raymond E, Gwizdek C, Dhib G, Poupon G, et al. Physiological and pathophysiological control of synaptic GluN2B-NMDA receptors by the C-terminal domain of amyloid precursor protein. elife. 2017;6: pubmed publisher

product information

Catalog Number :

107544

Product Name :

pAAV-AICD-NES-IRES-hrGFP

article :

doi	10.7554/eLife.25659
id	28192794
pubmed_id	28682239

bacterial resistance :

Ampicillin

cloning :

backbone

pAAV-IRES-hrGFP

backbone_mutation

backbone_origin

Agilent Technologies

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

AAV

growth notes :

Please note: Flag tag is not in frame with the insert and the left ITR has been altered but does not perturb virus production and expression.

growth strain :

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Export Signal (NES: CTCCCTCCACTAGAGCGACTAACCTTA) at C-term end and IRES-mediated co-expression of hrGFP

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	BamHI
promoter	CMV
sequencing_primer_3
sequencing_primer_5	ATTCTGAGTCCAAGCTAGGC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4
gene	APP
id	351

genbank_ids

mutation

None

name

AICD-NES

shRNA_sequence

size

species

9606

Homo sapiens