eMscL G22S | Addgene 107455 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

eMscL G22S

catalog :

107455

citations: 2

Reference
Cadoni S, Demen xe9 C, Alcala I, Provansal M, Nguyen D, Nelidova D, et al. Ectopic expression of a mechanosensitive channel confers spatiotemporal resolution to ultrasound stimulations of neurons for visual restoration. Nat Nanotechnol. 2023;18:667-676 pubmed publisher
Soloperto A, Boccaccio A, Contestabile A, Moroni M, Hallinan G, Palazzolo G, et al. Mechano-sensitization of mammalian neuronal networks through expression of the bacterial large-conductance mechanosensitive ion channel. J Cell Sci. 2018;131: pubmed publisher

product information

Catalog Number :

107455

Product Name :

eMscL G22S

article :

doi	10.1242/jcs.210393
id	28192782
pubmed_id	29361543

bacterial resistance :

Ampicillin

cloning :

backbone

pAAV2

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Other

Adeno-associated viral

growth strain :

eMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif

origin :

pi :

alt_names

mscL

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	EcoRI
promoter	human synapsin 1
sequencing_primer_3
sequencing_primer_5	5 - CTGCCTCAGTCTGCGGTGGG - 3
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	b3291, ECK3277, yhdC
gene	mscL
id	947787

genbank_ids

mutation

Bearing a glycine to serine substitution at position 22

name

bacterial mechanosensitive ion channel of large conductance

shRNA_sequence

size

411

species

32644

Other

Escherichia coli