This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pYES2.1-cerMET5
catalog :
107437
citations: 1
Reference
Reeves T, Warner D, Ludlow L, O Connor C. Pathways over Time: Functional Genomics Research in an Introductory Laboratory Course. CBE Life Sci Educ. 2018;17: pubmed publisher
product information
Catalog Number :
107437
Product Name :
pYES2.1-cerMET5
article :
doi10.1187/cbe.17-01-0012
id28192781
pubmed_id29326101
bacterial resistance :
Ampicillin
cloning :
backbonepYES2.1
backbone_mutation
backbone_originInvitrogen
backbone_size5886
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
contains the coding sequence for the beta subunit of sulfite reductase Construct was not functional in complementation analysis, but this may be due to tags rather than mutations in the coding sequence. Use with caution.
growth strain :
Overexpression of MET fusion protein in S. cerevisiae
origin :
37
pi :
alt_names
ECM17
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoterGAL1
sequencing_primer_3ACCGAGGAGAGGGTTAGGGAT
sequencing_primer_5AATATACCTCTATACTTTAACGTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYJR137C, ECM17
geneMET5
id853602
genbank_ids
mutationV81I, K341E, P597L, Y605C, E741G, D932G, N1217S, H1335R
nameMET5
shRNA_sequence
size4326
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on insert
tagV5-His
resistance markers :
3795
tags :
High Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA