This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
500 pSG5L HA RB del685 (NAAIRS)
catalog :
10729
citations: 1
Reference
Sellers W, Novitch B, Miyake S, Heith A, Otterson G, Kaye F, et al. Stable binding to E2F is not required for the retinoblastoma protein to activate transcription, promote differentiation, and suppress tumor cell growth. Genes Dev. 1998;12:95-106 pubmed
product information
Catalog Number :
10729
Product Name :
500 pSG5L HA RB del685 (NAAIRS)
article :
doi10.1101/gad.12.1.95
id369
pubmed_id9420334
bacterial resistance :
Ampicillin
cloning :
backbonepSG5L
backbone_mutation
backbone_origin
backbone_size4000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
ATCTGGACCCTTTTCaatgctgctatacgatcgGAGTAT
GAACTCATG
del685 created by site-directed mutagenesis using this primer:
origin :
37
pi :
alt_names
retinoblastoma
RB
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesOSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110
geneRB1
id5925
genbank_ids
mutationresidues 685 to 690 were replaced by NAAIRS, a flexible polylinker
nameRB del685
shRNA_sequence
size2800
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHA
resistance markers :
455
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA