This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MBP-rPKL S12TAG
catalog :
107155
citations: 1
Reference
Gassaway B, Cardone R, Padyana A, Petersen M, Judd E, Hayes S, et al. Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production. Cell Rep. 2019;29:3394-3404.e9 pubmed publisher
product information
Catalog Number :
107155
Product Name :
MBP-rPKL S12TAG
article :
doi10.1016/j.celrep.2019.11.009
id28192728
pubmed_id31825824
bacterial resistance :
Ampicillin
cloning :
backbonePCRT7
backbone_mutation
backbone_origin
backbone_size4467
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
MBP-rPKL construct for production and purification of rat PKL pS12
origin :
37
pi :
alt_names
rPKL
cloning
clone_methodRestriction Enzyme
cloning_site_3SacI
cloning_site_5NdeI
promoterPBAD
sequencing_primer_3
sequencing_primer_5CCTACCTGACGCTTTTTATCGCAACTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPK1, PKL, Pklg
genePklr
id24651
genbank_ids
mutationSerine 12 to TAG for phosphoserine insertion
nameMBP-tagged rat pyruvate kinase liver isoform
shRNA_sequence
size2810
species
10116
Rattus norvegicus
tags
location
tagC-term 6-his
location
tagN-term MBP
resistance markers :
1137
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA