PGEX-6P1-hNF2-AR mutant | Addgene 107149 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PGEX-6P1-hNF2-AR mutant

catalog :

107149

citations: 1

Reference
Chinthalapudi K, Mandati V, Zheng J, Sharff A, Bricogne G, Griffin P, et al. Lipid binding promotes the open conformation and tumor-suppressive activity of neurofibromin 2. Nat Commun. 2018;9:1338 pubmed publisher

product information

Catalog Number :

107149

Product Name :

PGEX-6P1-hNF2-AR mutant

article :

doi	10.1038/s41467-018-03648-4
id	28192724
pubmed_id	29626191

bacterial resistance :

Ampicillin

cloning :

backbone

pGEX-6P1

backbone_mutation

backbone_origin

GE Life Sciences

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

GST tag in frame with NF2 gene Note: The NFS insert contains A190V and G302E mutations compared to the closest reference sequence (NP_000259.1). The depositor has confirmed that these do not affect plasmid function.

growth strain :

bacterial expression

origin :

pi :

alt_names

NF2

SCH

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	Ptac
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ACN, BANF, SCH, SWNV, merlin-1
gene	NF2
id	4771

genbank_ids

mutation

A585W-R588K

name

neurofibromin 2

shRNA_sequence

size

1785

species

9606

Homo sapiens