This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET32a-Trx-PP-hNF2
catalog :
107148
citations: 2
Reference
Primi M, Rangarajan E, Patil D, Izard T. Conformational flexibility determines the Nf2/merlin tumor suppressor functions. Matrix Biol Plus. 2021;12:100074 pubmed publisher
Chinthalapudi K, Mandati V, Zheng J, Sharff A, Bricogne G, Griffin P, et al. Lipid binding promotes the open conformation and tumor-suppressive activity of neurofibromin 2. Nat Commun. 2018;9:1338 pubmed publisher
product information
Catalog Number :
107148
Product Name :
pET32a-Trx-PP-hNF2
article :
doi10.1038/s41467-018-03648-4
id28192724
pubmed_id29626191
bacterial resistance :
Ampicillin
cloning :
backbonepET32a
backbone_mutation
backbone_originNovagen (EMD Millipore)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Prescission protease site is inserted in frame with NF2 gene Note: The NFS insert contains A190V and G302E mutations compared to the closest reference sequence (NP_000259.1). The depositor has confirmed that these do not affect plasmid function.
growth strain :
bacterial expression
origin :
37
pi :
alt_names
NF2
SCH
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACN, BANF, SCH, SWNV, merlin-1
geneNF2
id4771
genbank_ids
mutation
name neurofibromin 2
shRNA_sequence
size1785
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag(His)6-Thioredoxin
resistance markers :
3774
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA