pLVET-HA-K-RasG12V-IRES-GFP | Addgene 107140 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLVET-HA-K-RasG12V-IRES-GFP

catalog :

107140

citations: 3

Reference
Herdy J, Traxler L, Agarwal R, Karbacher L, Schlachetzki J, Boehnke L, et al. Increased post-mitotic senescence in aged human neurons is a pathological feature of Alzheimer's disease. Cell Stem Cell. 2022;29:1637-1652.e6 pubmed publisher
Gupta A, Lu D, Balasubramanian H, Chi Z, Wohland T. Heptanol-mediated phase separation determines phase preference of molecules in live cell membranes. J Lipid Res. 2022;63:100220 pubmed publisher
Zhang K, MyllymÃ¤ki S, Gao P, Devarajan R, KytÃ¶lÃ¤ V, Nykter M, et al. Oncogenic K-Ras upregulates ITGA6 expression via FOSL1 to induce anoikis resistance and synergizes with Î±V-Class integrins to promote EMT. Oncogene. 2017;36:5681-5694 pubmed publisher

product information

Catalog Number :

107140

Product Name :

pLVET-HA-K-RasG12V-IRES-GFP

article :

doi	10.1038/onc.2017.177
id	28192718
pubmed_id	28604746

bacterial resistance :

Ampicillin

cloning :

backbone

pLVET-IRES-GFP

backbone_mutation

backbone_origin

backbone_size

11261

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

K-RasG12V PCR:ed from pLenti-PGK-KRAS4B(G12V)(Addgene #35633) and ligated as PmeI/BamHI fragment into pLVET-IRES-GFP

growth strain :

A bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFP

origin :

pi :

alt_names

K-Ras

EGFP

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	PmeI
promoter	hEF-1alpha
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
gene	KRAS
id	3845

genbank_ids

mutation

name

HA-tagged Kras4B(G12V) and GFP

shRNA_sequence

size

597

species

9606

Homo sapiens

32644

Other